TY - JOUR
T1 - IL-7 enhances Ag-specific human T cell response by increasing expression of IL-2R α and γ chains
AU - Chou, Yuan K.
AU - Bourdette, Dennis N.
AU - Barnes, David
AU - Finn, Thomas P.
AU - Murray, Susan
AU - Unsicker, Laura
AU - Robey, Ian
AU - Whitham, Ruth
AU - Buenafe, Abigail C.
AU - Allegretta, Mark
AU - Offner, Halina
AU - Vandenbark, Arthur A.
N1 - Funding Information:
The authors thank Shelley Cooper for arranging patient visits and drawing blood, Eva Niehaus for assistance in preparation of the manuscript, and Dr. Raymond Goodwin of Immunex for reviewing the manuscript. This work was supported by the Department of Veterans Affairs, Grants NS23221 and NS23444 from the National Institutes of Health, the Nancy Davis Center Without Walls, and private donors.
PY - 1999/4/1
Y1 - 1999/4/1
N2 - Interleukin-7 has demonstrated potent enhancing effects on the growth and differentiation of several immature celt types, including thymocytes, and on survival of resting and antigen activated T cells. In this study, we evaluated the effects of IL-7 on post-thymic antigen-specific T cells from human blood. IL-7 was found to enhance proliferation responses and IFN-γ secretion of myelin or recall Ag-specific Th1 cells through the selective up- regulation of the IL-2Rα and γ but not β chains in both an Ag-dependent and Ag-independent manner, but did not affect monocytes, B cells, or NK cells. These functions of IL-7 enhanced the detection of Th1 but not Th2 cell frequency by > 2.5 fold, and promoted selection of Ag-specific Th1 cells by the limiting dilution method. Moreover, IL-7 pretreatment conferred increased resistance of CD4 + T cells to CD8 + cell lysis. These studies demonstrate that IL-7 promotes the growth and survival of circulating Ag-specific human Th1 cells through a mechanism that probably involves the γc common receptor for IL-2 family members that includes IL-7.
AB - Interleukin-7 has demonstrated potent enhancing effects on the growth and differentiation of several immature celt types, including thymocytes, and on survival of resting and antigen activated T cells. In this study, we evaluated the effects of IL-7 on post-thymic antigen-specific T cells from human blood. IL-7 was found to enhance proliferation responses and IFN-γ secretion of myelin or recall Ag-specific Th1 cells through the selective up- regulation of the IL-2Rα and γ but not β chains in both an Ag-dependent and Ag-independent manner, but did not affect monocytes, B cells, or NK cells. These functions of IL-7 enhanced the detection of Th1 but not Th2 cell frequency by > 2.5 fold, and promoted selection of Ag-specific Th1 cells by the limiting dilution method. Moreover, IL-7 pretreatment conferred increased resistance of CD4 + T cells to CD8 + cell lysis. These studies demonstrate that IL-7 promotes the growth and survival of circulating Ag-specific human Th1 cells through a mechanism that probably involves the γc common receptor for IL-2 family members that includes IL-7.
KW - Human T cells
KW - IL-2R
KW - IL-7
KW - Survival factor
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U2 - 10.1016/S0165-5728(99)00002-8
DO - 10.1016/S0165-5728(99)00002-8
M3 - Article
C2 - 10227429
AN - SCOPUS:0033119580
VL - 96
SP - 101
EP - 111
JO - Advances in Neuroimmunology
JF - Advances in Neuroimmunology
SN - 0165-5728
IS - 1
ER -