TY - JOUR
T1 - IL-7 enhances Ag-specific human T cell response by increasing expression of IL-2R α and γ chains
AU - Chou, Yuan K.
AU - Bourdette, Dennis N.
AU - Barnes, David
AU - Finn, Thomas P.
AU - Murray, Susan
AU - Unsicker, Laura
AU - Robey, Ian
AU - Whitham, Ruth H.
AU - Buenafe, Abigail C.
AU - Allegretta, Mark
AU - Offner, Halina
AU - Vandenbark, Arthur A.
PY - 1999/4/1
Y1 - 1999/4/1
N2 - Interleukin-7 has demonstrated potent enhancing effects on the growth and differentiation of several immature celt types, including thymocytes, and on survival of resting and antigen activated T cells. In this study, we evaluated the effects of IL-7 on post-thymic antigen-specific T cells from human blood. IL-7 was found to enhance proliferation responses and IFN-γ secretion of myelin or recall Ag-specific Th1 cells through the selective up- regulation of the IL-2Rα and γ but not β chains in both an Ag-dependent and Ag-independent manner, but did not affect monocytes, B cells, or NK cells. These functions of IL-7 enhanced the detection of Th1 but not Th2 cell frequency by > 2.5 fold, and promoted selection of Ag-specific Th1 cells by the limiting dilution method. Moreover, IL-7 pretreatment conferred increased resistance of CD4 + T cells to CD8 + cell lysis. These studies demonstrate that IL-7 promotes the growth and survival of circulating Ag-specific human Th1 cells through a mechanism that probably involves the γc common receptor for IL-2 family members that includes IL-7.
AB - Interleukin-7 has demonstrated potent enhancing effects on the growth and differentiation of several immature celt types, including thymocytes, and on survival of resting and antigen activated T cells. In this study, we evaluated the effects of IL-7 on post-thymic antigen-specific T cells from human blood. IL-7 was found to enhance proliferation responses and IFN-γ secretion of myelin or recall Ag-specific Th1 cells through the selective up- regulation of the IL-2Rα and γ but not β chains in both an Ag-dependent and Ag-independent manner, but did not affect monocytes, B cells, or NK cells. These functions of IL-7 enhanced the detection of Th1 but not Th2 cell frequency by > 2.5 fold, and promoted selection of Ag-specific Th1 cells by the limiting dilution method. Moreover, IL-7 pretreatment conferred increased resistance of CD4 + T cells to CD8 + cell lysis. These studies demonstrate that IL-7 promotes the growth and survival of circulating Ag-specific human Th1 cells through a mechanism that probably involves the γc common receptor for IL-2 family members that includes IL-7.
KW - Human T cells
KW - IL-2R
KW - IL-7
KW - Survival factor
UR - http://www.scopus.com/inward/record.url?scp=0033119580&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033119580&partnerID=8YFLogxK
U2 - 10.1016/S0165-5728(99)00002-8
DO - 10.1016/S0165-5728(99)00002-8
M3 - Article
C2 - 10227429
AN - SCOPUS:0033119580
VL - 96
SP - 101
EP - 111
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1
ER -