IL-2 secretion is pertussis toxin sensitive in a T lymphocyte hybridoma

J. B. Stanley, R. M. Gorczynski, T. L. Delovitch, Gordon Mills

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Interaction of specific ligands with TCR initiates a cascade of biochemical events which leads to expression of high affinity IL-2R and subsequent IL-2 secretion. Activation of phospholipase C (PL-C) is considered to be a key event in the initiation of this cascade. However, in addition to this PL-C-dependent pathway, PL-C-independent pathways have been hypothesized. Identification of the steps constituting these PL-C-independent pathways has been difficult because activation of PL-C and the subsequent cascade of events mask the effects of such pathways. Specific inhibitors for PL-C, or mutants defective in, the PL-C pathway would facilitate delineation of alternative activation pathways. We have identified a murine pork insulin/IA(d)-specific T cell hybridoma, B8P3.11, in which perturbation of the B8P3.11 TCR by either Ag in association with Ia, anti-CD3 antibodies, or a mitogenic lectin does not induce increases in myo-inositol 1,4,5-triphosphate production or cystolic free calcium, yet it does lead to IL-2 secretion. Treatment of B8P3.11 with pertussis toxin, at concentrations which ADP-ribosylate GTP-binding proteins, inhibits IL-2 secretion. Thus, signal transduction resulting in IL-2 secretion by B8P3.11 likely involves G protein. In contrast, TCR/ligand interaction activates the PL-C-dependent pathway in LBRM 331A5, a T cell lymphoma. Furthermore, pertussis toxin treatment, which blocks IL-2 secretion by B8P3.11, does not alter IL-2 secretion by LBRM 331A5. However, similar pertussis toxin substrates are present in both cells. Therefore, B8P3.11 T cells should help to elucidate PL-C-independent activation pathways.

Original languageEnglish (US)
Pages (from-to)3546-3552
Number of pages7
JournalJournal of Immunology
Volume142
Issue number10
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Pertussis Toxin
Hybridomas
Type C Phospholipases
Interleukin-2
T-Lymphocytes
GTP-Binding Proteins
Ligands
Inositol 1,4,5-Trisphosphate
T-Cell Lymphoma
Masks
Lectins
Adenosine Diphosphate
Anti-Idiotypic Antibodies
Signal Transduction
Insulin
Calcium

ASJC Scopus subject areas

  • Immunology

Cite this

Stanley, J. B., Gorczynski, R. M., Delovitch, T. L., & Mills, G. (1989). IL-2 secretion is pertussis toxin sensitive in a T lymphocyte hybridoma. Journal of Immunology, 142(10), 3546-3552.

IL-2 secretion is pertussis toxin sensitive in a T lymphocyte hybridoma. / Stanley, J. B.; Gorczynski, R. M.; Delovitch, T. L.; Mills, Gordon.

In: Journal of Immunology, Vol. 142, No. 10, 01.01.1989, p. 3546-3552.

Research output: Contribution to journalArticle

Stanley, JB, Gorczynski, RM, Delovitch, TL & Mills, G 1989, 'IL-2 secretion is pertussis toxin sensitive in a T lymphocyte hybridoma', Journal of Immunology, vol. 142, no. 10, pp. 3546-3552.
Stanley JB, Gorczynski RM, Delovitch TL, Mills G. IL-2 secretion is pertussis toxin sensitive in a T lymphocyte hybridoma. Journal of Immunology. 1989 Jan 1;142(10):3546-3552.
Stanley, J. B. ; Gorczynski, R. M. ; Delovitch, T. L. ; Mills, Gordon. / IL-2 secretion is pertussis toxin sensitive in a T lymphocyte hybridoma. In: Journal of Immunology. 1989 ; Vol. 142, No. 10. pp. 3546-3552.
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