IL-1 Receptor–Knockout Mice Develop Epidermal Cysts and Show an Altered Innate Immune Response after Exposure to UVB Radiation

Nikhil N. Kulkarni, Christopher A. Adase, Ling juan Zhang, Andrew W. Borkowski, Fengwu Li, James A. Sanford, Daniel J. Coleman, Carlos Aguilera, Arup K. Indra, Richard L. Gallo

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

In this study, we observed that mice lacking the IL-1 receptor (IL-1R) (IL1r–/–) or deficient in IL1-β developed multiple epidermal cysts after chronic UVB exposure. Cysts that developed in IL1r–/– mice were characterized by the presence of the hair follicle marker Sox 9, keratins 10 and 14, and normal melanocyte distribution and retinoid X receptor-α expression. The increased incidence of cysts in IL1r–/– mice was associated with less skin inflammation as characterized by decreased recruitment of macrophages, and their skin also maintained epidermal barrier function compared with wild-type mice. Transcriptional analysis of the skin of IL1r–/– mice after UVB exposure showed decreased gene expression of proinflammatory cytokines such as tumor necrosis factor-α and IL-6. In vitro, primary keratinocytes derived from IL1r–/– mice were more resistant to UVB-triggered cell death compared with wild-type cells, and tumor necrosis factor-α release was completely blocked in the absence of IL-1R. These observations illustrate an unexpected yet prominent phenotype associated with the lack of IL-1R signaling in mice and support further investigation into the role of IL-1 ligands in epidermal repair and innate immune response after damaging UVB exposure.

Original languageEnglish (US)
Pages (from-to)2417-2426
Number of pages10
JournalJournal of Investigative Dermatology
Volume137
Issue number11
DOIs
StatePublished - Nov 2017
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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