IgG B memory cell subpopulations: Differences in susceptibility to stimulation by TI-1 and TI-2 antigens

T. V. Tittle, M. B. Rittenberg

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Spleen cells from mice primed with the thymus dependent (TD) antigen trinitrophenyl keyhole limpet hemocyanin several months earlier can be stimulated in vitro to produce an IgG anti-hapten response to TD as well as thymus independent (TI) forms of the hapten. Selective killing of TD or TI-2 responding B cells can be accomplished with the corresponding antigen by bromouridine deoxyribose (BUdR) and light treatment without affecting the other population. In contrast, we show here that selective killing with TI-1 antigens does not occur. Rather, the TI-1 antigens, TNP-Brucella abortus or TNP-lipopolysaccharide, eliminate both TD and TI-2 responding IgG memory B cells. All TNP-responding B cells are similarly eliminated if cultures are challenged simultaneously with TD and TI-2 antigens before BUdR and light but not when they are challenged with either a TD or TI-2 antigen separately. We conclude that IgG memory B cell precursors stimulated to produce anti-TNP by TD or TI-2 forms of the hapten are defined by only two functionally distinct subpopulations and that TI-1 antigens can stimulate both of these populations at least to divide.

Original languageEnglish (US)
Pages (from-to)202-206
Number of pages5
JournalJournal of Immunology
Volume124
Issue number1
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'IgG B memory cell subpopulations: Differences in susceptibility to stimulation by TI-1 and TI-2 antigens'. Together they form a unique fingerprint.

  • Cite this