IFN-I and IL-22 mediate protective effects of intestinal viral infection

Jessica A. Neil, Yu Matsuzawa-Ishimoto, Elisabeth Kernbauer-Hölzl, Samantha L. Schuster, Stela Sota, Mericien Venzon, Simone Dallari, Antonio Galvao Neto, Ashley Hine, David Hudesman, P’ng Loke, Timothy Nice, Ken Cadwell

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Products derived from bacterial members of the gut microbiota evoke immune signalling pathways of the host that promote immunity and barrier function in the intestine. How immune reactions to enteric viruses support intestinal homeostasis is unknown. We recently demonstrated that infection by murine norovirus (MNV) reverses intestinal abnormalities following depletion of bacteria, indicating that an intestinal animal virus can provide cues to the host that are typically attributed to the microbiota. Here, we elucidate mechanisms by which MNV evokes protective responses from the host. We identify an important role for the viral protein NS1/2 in establishing local replication and a type I interferon (IFN-I) response in the colon. We further show that IFN-I acts on intestinal epithelial cells to increase the proportion of CCR2-dependent macrophages and interleukin (IL)-22-producing innate lymphoid cells, which in turn promote pSTAT3 signalling in intestinal epithelial cells and protection from intestinal injury. In addition, we demonstrate that MNV provides a striking IL-22-dependent protection against early-life lethal infection by Citrobacter rodentium. These findings demonstrate novel ways in which a viral member of the microbiota fortifies the intestinal barrier during chemical injury and infectious challenges.

Original languageEnglish (US)
JournalNature Microbiology
DOIs
StatePublished - Jan 1 2019

Fingerprint

Norovirus
Virus Diseases
Citrobacter rodentium
Epithelial Cells
Interferon Type I
Cytoprotection
Enterovirus
Microbiota
Wounds and Injuries
Viral Proteins
Infection
Intestines
Cues
Immunity
Colon
Homeostasis
Macrophages
Lymphocytes
Viruses
Bacteria

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Genetics
  • Microbiology (medical)
  • Cell Biology

Cite this

Neil, J. A., Matsuzawa-Ishimoto, Y., Kernbauer-Hölzl, E., Schuster, S. L., Sota, S., Venzon, M., ... Cadwell, K. (2019). IFN-I and IL-22 mediate protective effects of intestinal viral infection. Nature Microbiology. https://doi.org/10.1038/s41564-019-0470-1

IFN-I and IL-22 mediate protective effects of intestinal viral infection. / Neil, Jessica A.; Matsuzawa-Ishimoto, Yu; Kernbauer-Hölzl, Elisabeth; Schuster, Samantha L.; Sota, Stela; Venzon, Mericien; Dallari, Simone; Galvao Neto, Antonio; Hine, Ashley; Hudesman, David; Loke, P’ng; Nice, Timothy; Cadwell, Ken.

In: Nature Microbiology, 01.01.2019.

Research output: Contribution to journalArticle

Neil, JA, Matsuzawa-Ishimoto, Y, Kernbauer-Hölzl, E, Schuster, SL, Sota, S, Venzon, M, Dallari, S, Galvao Neto, A, Hine, A, Hudesman, D, Loke, P, Nice, T & Cadwell, K 2019, 'IFN-I and IL-22 mediate protective effects of intestinal viral infection', Nature Microbiology. https://doi.org/10.1038/s41564-019-0470-1
Neil JA, Matsuzawa-Ishimoto Y, Kernbauer-Hölzl E, Schuster SL, Sota S, Venzon M et al. IFN-I and IL-22 mediate protective effects of intestinal viral infection. Nature Microbiology. 2019 Jan 1. https://doi.org/10.1038/s41564-019-0470-1
Neil, Jessica A. ; Matsuzawa-Ishimoto, Yu ; Kernbauer-Hölzl, Elisabeth ; Schuster, Samantha L. ; Sota, Stela ; Venzon, Mericien ; Dallari, Simone ; Galvao Neto, Antonio ; Hine, Ashley ; Hudesman, David ; Loke, P’ng ; Nice, Timothy ; Cadwell, Ken. / IFN-I and IL-22 mediate protective effects of intestinal viral infection. In: Nature Microbiology. 2019.
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