Identification of three new microsatellite markers in the spinocerebellar ataxia type 2 (SCA2) region and 1.2 Mb physical map

Tamilla Nechiporuk, Alex Nechiporuk, Xiaoping Guan, Richard Frederick, Karla Figueroa, Ilya Chumakov, Julie R. Korenberg, Pieter J. De Jong, Stefan M. Pulst

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Spinocerebellar ataxia type 2 (SCA2) is a neurodegenerative disease recently mapped to chromosome 12q close to the locus D12S84 by genetic linkage analysis. To generate additional genetic markers in the SCA2 region, we constructed a physical map of the region using yeast artificial chomosome (YAC), P1 artificial chromosome (PAC) and cosmid clones. The physical map was found to agree well with the genetic map. Three novel microsatellite markers were isolated and physically mapped. A novel approach to isolate CAG repeats directly from YAC DNAs is described.

Original languageEnglish (US)
Pages (from-to)462-467
Number of pages6
JournalHuman genetics
Volume97
Issue number4
DOIs
StatePublished - Apr 1996

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ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Nechiporuk, T., Nechiporuk, A., Guan, X., Frederick, R., Figueroa, K., Chumakov, I., Korenberg, J. R., De Jong, P. J., & Pulst, S. M. (1996). Identification of three new microsatellite markers in the spinocerebellar ataxia type 2 (SCA2) region and 1.2 Mb physical map. Human genetics, 97(4), 462-467. https://doi.org/10.1007/BF02267067