Identification of three new microsatellite markers in the spinocerebellar ataxia type 2 (SCA2) region and 1.2 Mb physical map

Tamilla Nechiporuk; Alex Nechiporuk; Xiaoping Guan; Richard Frederick; Karla Figueroa; Ilya Chumakov; Julie R. Korenberg; Pieter J. De Jong; Stefan M. Pulst

doi:10.1007/BF02267067

Identification of three new microsatellite markers in the spinocerebellar ataxia type 2 (SCA2) region and 1.2 Mb physical map

Tamilla Nechiporuk, Alex Nechiporuk, Xiaoping Guan, Richard Frederick, Karla Figueroa, Ilya Chumakov, Julie R. Korenberg, Pieter J. De Jong, Stefan M. Pulst

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Spinocerebellar ataxia type 2 (SCA2) is a neurodegenerative disease recently mapped to chromosome 12q close to the locus D12S84 by genetic linkage analysis. To generate additional genetic markers in the SCA2 region, we constructed a physical map of the region using yeast artificial chomosome (YAC), P1 artificial chromosome (PAC) and cosmid clones. The physical map was found to agree well with the genetic map. Three novel microsatellite markers were isolated and physically mapped. A novel approach to isolate CAG repeats directly from YAC DNAs is described.

Original language	English (US)
Pages (from-to)	462-467
Number of pages	6
Journal	Human genetics
Volume	97
Issue number	4
DOIs	https://doi.org/10.1007/BF02267067
State	Published - Apr 1996
Externally published	Yes

ASJC Scopus subject areas

Genetics
Genetics(clinical)

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title = "Identification of three new microsatellite markers in the spinocerebellar ataxia type 2 (SCA2) region and 1.2 Mb physical map",

abstract = "Spinocerebellar ataxia type 2 (SCA2) is a neurodegenerative disease recently mapped to chromosome 12q close to the locus D12S84 by genetic linkage analysis. To generate additional genetic markers in the SCA2 region, we constructed a physical map of the region using yeast artificial chomosome (YAC), P1 artificial chromosome (PAC) and cosmid clones. The physical map was found to agree well with the genetic map. Three novel microsatellite markers were isolated and physically mapped. A novel approach to isolate CAG repeats directly from YAC DNAs is described.",

author = "Tamilla Nechiporuk and Alex Nechiporuk and Xiaoping Guan and Richard Frederick and Karla Figueroa and Ilya Chumakov and Korenberg, {Julie R.} and {De Jong}, {Pieter J.} and Pulst, {Stefan M.}",

year = "1996",

month = apr,

doi = "10.1007/BF02267067",

language = "English (US)",

volume = "97",

pages = "462--467",

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T1 - Identification of three new microsatellite markers in the spinocerebellar ataxia type 2 (SCA2) region and 1.2 Mb physical map

AU - Nechiporuk, Tamilla

AU - Nechiporuk, Alex

AU - Guan, Xiaoping

AU - Frederick, Richard

AU - Figueroa, Karla

AU - Chumakov, Ilya

AU - Korenberg, Julie R.

AU - De Jong, Pieter J.

AU - Pulst, Stefan M.

PY - 1996/4

Y1 - 1996/4

N2 - Spinocerebellar ataxia type 2 (SCA2) is a neurodegenerative disease recently mapped to chromosome 12q close to the locus D12S84 by genetic linkage analysis. To generate additional genetic markers in the SCA2 region, we constructed a physical map of the region using yeast artificial chomosome (YAC), P1 artificial chromosome (PAC) and cosmid clones. The physical map was found to agree well with the genetic map. Three novel microsatellite markers were isolated and physically mapped. A novel approach to isolate CAG repeats directly from YAC DNAs is described.

AB - Spinocerebellar ataxia type 2 (SCA2) is a neurodegenerative disease recently mapped to chromosome 12q close to the locus D12S84 by genetic linkage analysis. To generate additional genetic markers in the SCA2 region, we constructed a physical map of the region using yeast artificial chomosome (YAC), P1 artificial chromosome (PAC) and cosmid clones. The physical map was found to agree well with the genetic map. Three novel microsatellite markers were isolated and physically mapped. A novel approach to isolate CAG repeats directly from YAC DNAs is described.

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Identification of three new microsatellite markers in the spinocerebellar ataxia type 2 (SCA2) region and 1.2 Mb physical map

Abstract

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