Identification of the tyrosine phosphatase PTPlC as a B cell antigen receptor-associated protein involved in the regulation of B cell signaling

Giovanni Pani, Maya Kozlowski, John C. Cambier, Gordon Mills, Katherine A. Siminovitch

Research output: Contribution to journalArticle

230 Citations (Scopus)

Abstract

Recent data implicating loss of PTPIC tyrosine phosphatase activity in the genesis of the multiple hemopoietic cell defects found in systemic autoimmune/immunodeficient motheaten (me) and viable motheaten (mev) mice suggest that PTP1C plays an important role in modulating intracellular signaling events regulating cell activation and differentiation. To begin elucidating the role for this cytosolic phosphatase in lymphoid cell signal transduction, we have examined early signaling events and mitogenic responses induced by B cell antigen receptor (BCK) ligation in me and mev splenic B cells and in CD5 + CH12 lymphoma cells, which represent the lymphoid population amplified in motheaten mice. Despite their lack of functional Irl’PlC, me and me -B cells proliferated normally in response to LPS. However, compared with wild-type B cells, cells from the mutant mice were hyperresponsive to normally submitogenic concentrations of F(ab’)2 anti-Ig antibody, and they exhibited reduced susceptibility to the inhibitory effects of FcγIIRB cross-linking on BCR-induced proliferation. Additional studies of unstimulated CH12 and wild-type splenic B cells revealed the constitutive association of PTPIC with the resting BCR complex, as evidenced by coprecipitation of PTPIC protein and phosphatase activity with BCR components and the depletion of BCK-associated tyrosine phosphatase activity by anti- PTPIC antibodies. These results suggest a role for trl’PlC in regulating the tyrosine phosphorylation state of the resting BCR complex components, a hypothesis supported by the observation that PTP1C specifically induces dephosphorylation of a 35-kD BCR-associated protein likely representing Ig-ot. In contrast, whereas membrane Ig cross-linking was associated with an increase in the tyrosine phosphorylation of PTP1C and an ~140-kD coprecipitated protein, PTP1C was no longer detected in the BCR complex after receptor engagement, suggesting that PTP1C dissociates from the activated receptor complex. Together these results suggest a critical role for PTP1C in modulating BCR signaling capacity, and they indicate that the PTP1C influence on B cell signaling is likely to be realized in both resting and activated cells.

Original languageEnglish (US)
Pages (from-to)2077-2084
Number of pages8
JournalJournal of Experimental Medicine
Volume181
Issue number6
DOIs
StatePublished - Jun 1 1995
Externally publishedYes

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B-Cell Antigen Receptors
Phosphoric Monoester Hydrolases
Tyrosine
B-Lymphocytes
Proteins
Anti-Idiotypic Antibodies
Phosphorylation
Phosphoprotein Phosphatases
Ligation
Cell Differentiation
Signal Transduction
Lymphoma
Lymphocytes
Membranes
Population

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Identification of the tyrosine phosphatase PTPlC as a B cell antigen receptor-associated protein involved in the regulation of B cell signaling. / Pani, Giovanni; Kozlowski, Maya; Cambier, John C.; Mills, Gordon; Siminovitch, Katherine A.

In: Journal of Experimental Medicine, Vol. 181, No. 6, 01.06.1995, p. 2077-2084.

Research output: Contribution to journalArticle

Pani, Giovanni ; Kozlowski, Maya ; Cambier, John C. ; Mills, Gordon ; Siminovitch, Katherine A. / Identification of the tyrosine phosphatase PTPlC as a B cell antigen receptor-associated protein involved in the regulation of B cell signaling. In: Journal of Experimental Medicine. 1995 ; Vol. 181, No. 6. pp. 2077-2084.
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abstract = "Recent data implicating loss of PTPIC tyrosine phosphatase activity in the genesis of the multiple hemopoietic cell defects found in systemic autoimmune/immunodeficient motheaten (me) and viable motheaten (mev) mice suggest that PTP1C plays an important role in modulating intracellular signaling events regulating cell activation and differentiation. To begin elucidating the role for this cytosolic phosphatase in lymphoid cell signal transduction, we have examined early signaling events and mitogenic responses induced by B cell antigen receptor (BCK) ligation in me and mev splenic B cells and in CD5 + CH12 lymphoma cells, which represent the lymphoid population amplified in motheaten mice. Despite their lack of functional Irl’PlC, me and me -B cells proliferated normally in response to LPS. However, compared with wild-type B cells, cells from the mutant mice were hyperresponsive to normally submitogenic concentrations of F(ab’)2 anti-Ig antibody, and they exhibited reduced susceptibility to the inhibitory effects of FcγIIRB cross-linking on BCR-induced proliferation. Additional studies of unstimulated CH12 and wild-type splenic B cells revealed the constitutive association of PTPIC with the resting BCR complex, as evidenced by coprecipitation of PTPIC protein and phosphatase activity with BCR components and the depletion of BCK-associated tyrosine phosphatase activity by anti- PTPIC antibodies. These results suggest a role for trl’PlC in regulating the tyrosine phosphorylation state of the resting BCR complex components, a hypothesis supported by the observation that PTP1C specifically induces dephosphorylation of a 35-kD BCR-associated protein likely representing Ig-ot. In contrast, whereas membrane Ig cross-linking was associated with an increase in the tyrosine phosphorylation of PTP1C and an ~140-kD coprecipitated protein, PTP1C was no longer detected in the BCR complex after receptor engagement, suggesting that PTP1C dissociates from the activated receptor complex. Together these results suggest a critical role for PTP1C in modulating BCR signaling capacity, and they indicate that the PTP1C influence on B cell signaling is likely to be realized in both resting and activated cells.",
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