Identification of the minimal replication region of the multicopy Streptomyces plasmid pSL1

Yutaka Shindoh, Hiroaki Urabe, Michiko Nakano, Hiroshi Ogawara

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The 1.52-kb minimal replication origin of the 3.9-kb Streptomyces plasmid pSL1 was determined using a bifunctional derivative, pMCP44, of pSL1. Plasmids with linker insertions into the pSL1 part of pMCP44 were isolated from Escherichia coli. The sites of insertion were determined by restriction enzyme analysis and the ability of the mutant plasmids to replicate in S. lividans 66 was determined. All except one of the inserts in the 1.52-kb essential region inactivated replication. A 104-bp segment from this region could function as a replication origin in the presence of a helper plasmid containing a nonoverlapping pSL1 fragment. The sequence of this 104-bp fragment shows similarities to those of known plasmid replication origins.

Original languageEnglish (US)
Pages (from-to)149-156
Number of pages8
JournalPlasmid
Volume17
Issue number2
DOIs
Publication statusPublished - 1987
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Molecular Biology

Cite this