Identification of the intracellular gate for a member of the equilibrative nucleoside transporter (ENT) family

Raquel Valdés, Johannes Elferich, Ujwal Shinde, Scott Landfear

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Equilibrative nucleoside transporters of the SLC29 family play important roles in many physiological and pharmacological processes, including import of drugs for treatment of cancer, AIDS, cardiovascular, and parasitic diseases. However, no crystal structure is available for any member of this family. In previous studies we generated a computational model of the Leishmania donovani nucleoside transporter 1.1 (LdNT1.1) that captured this permease in the outward-closed conformation, and we identified the extracellular gate. In the present study we have modeled the inward-closed conformation of LdNT1.1 using the crystal structure of the Escherichia coli fucose transporter FucP and have identified four transmembrane helices whose ends close to form a predicted intracellular gate.Wehave tested this prediction by site-directed mutagenesis of relevant helix residues and by cross-linking of introduced cysteine pairs. The results are consistent with the predictions of the computational model and suggest that a similarly constituted gate operates in other members of the equilibrative nucleoside transporter family.

Original languageEnglish (US)
Pages (from-to)8799-8809
Number of pages11
JournalJournal of Biological Chemistry
Volume289
Issue number13
DOIs
StatePublished - Mar 30 2014

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Nucleoside Transport Proteins
Leishmania donovani
Conformations
Crystal structure
Physiological Phenomena
Mutagenesis
Parasitic Diseases
Fucose
Membrane Transport Proteins
Site-Directed Mutagenesis
Escherichia coli
Cysteine
Acquired Immunodeficiency Syndrome
Cardiovascular Diseases
Pharmaceutical Preparations
Neoplasms

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Identification of the intracellular gate for a member of the equilibrative nucleoside transporter (ENT) family. / Valdés, Raquel; Elferich, Johannes; Shinde, Ujwal; Landfear, Scott.

In: Journal of Biological Chemistry, Vol. 289, No. 13, 30.03.2014, p. 8799-8809.

Research output: Contribution to journalArticle

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