Identification of the antifungal peptide-containing symbiont of the marine sponge Theonella swinhoei as a novel δ-proteobacterium, "Candidatus Entotheonella palauensis"

E. W. Schmidt, A. Y. Obraztsova, S. K. Davidson, D. J. Faulkner, Margo Haygood

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

The Palauan sponge Theonella swinhoei (class Demospongiae, order Lithistida, family Theonellidae) harbors filamentous bacterial symbionts that contain theopalauamide, an antifungal, bicyclic glycopeptide. In this study, the filamentous symbionts were shown to be novel bacteria belonging to the δ-subdivision of proteobacteria. The 16S rRNA gene sequence was determined using a combination of denaturing gradient-gel electrophoresis (DGGE) and specific polymerase chain-reaction (PCR) primers, and its source was confirmed by in situ hybridization. In a series of culture experiments, the filamentous bacteria were propagated in a mixed culture on agar plates. Related 16S rRNA gene sequences were isolated from related sponges with slightly different chemistry. The taxonomic status "Candidatus Entotheonella palauensis" is proposed for the theopalauamide-containing filamentous bacteria from T. swinhoei.

Original languageEnglish (US)
Pages (from-to)969-977
Number of pages9
JournalMarine Biology
Volume136
Issue number6
StatePublished - 2000
Externally publishedYes

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Candidatus Entotheonella palauensis
antimicrobial peptides
Proteobacteria
Porifera
symbiont
sponge
peptide
symbionts
bacterium
bacteria
ribosomal RNA
glycopeptides
nucleotide sequences
gene
mixed culture
denaturing gradient gel electrophoresis
agar
in situ hybridization
polymerase chain reaction
electrokinesis

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Identification of the antifungal peptide-containing symbiont of the marine sponge Theonella swinhoei as a novel δ-proteobacterium, "Candidatus Entotheonella palauensis". / Schmidt, E. W.; Obraztsova, A. Y.; Davidson, S. K.; Faulkner, D. J.; Haygood, Margo.

In: Marine Biology, Vol. 136, No. 6, 2000, p. 969-977.

Research output: Contribution to journalArticle

Schmidt, E. W. ; Obraztsova, A. Y. ; Davidson, S. K. ; Faulkner, D. J. ; Haygood, Margo. / Identification of the antifungal peptide-containing symbiont of the marine sponge Theonella swinhoei as a novel δ-proteobacterium, "Candidatus Entotheonella palauensis". In: Marine Biology. 2000 ; Vol. 136, No. 6. pp. 969-977.
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