Identification of Synaptic Targets of Drosophila Pumilio

Gengxin Chen, Wanhe Li, Qing-Shuo Zhang, Michael Regulski, Nishi Sinha, Jody Barditch, Tim Tully, Adrian R. Krainer, Michael Q. Zhang, Josh Dubnau

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Drosophila Pumilio (Pum) protein is a translational regulator involved in embryonic patterning and germline development. Recent findings demonstrate that Pum also plays an important role in the nervous system, both at the neuromuscular junction (NMJ) and in long-term memory formation. In neurons, Pum appears to play a role in homeostatic control of excitability via down regulation of para, a voltage gated sodium channel, and may more generally modulate local protein synthesis in neurons via translational repression of eIF-4E. Aside from these, the biologically relevant targets of Pum in the nervous system remain largely unknown. We hypothesized that Pum might play a role in regulating the local translation underlying synapse-specific modifications during memory formation. To identify relevant translational targets, we used an informatics approach to predict Pum targets among mRNAs whose products have synaptic localization. We then used both in vitro binding and two in vivo assays to functionally confirm the fidelity of this informatics screening method. We find that Pum strongly and specifically binds to RNA sequences in the 3′UTR of four of the predicted target genes, demonstrating the validity of our method. We then demonstrate that one of these predicted target sequences, in the 3′UTR of discs large (dlg1), the Drosophila PSD95 ortholog, can functionally substitute for a canonical NRE (Nanos response element) in vivo in a heterologous functional assay. Finally, we show that the endogenous dlg1 mRNA can be regulated by Pumilio in a neuronal context, the adult mushroom bodies (MB), which is an anatomical site of memory storage.

Original languageEnglish (US)
Article numbere1000026
JournalPLoS Computational Biology
Volume4
Issue number2
DOIs
StatePublished - Feb 2008
Externally publishedYes

Fingerprint

Informatics
Drosophilidae
Nervous System
Drosophila
informatics
Neurology
Mushroom Bodies
nervous system
Voltage-Gated Sodium Channels
Neurons
Data storage equipment
Messenger RNA
Target
Long-Term Memory
Neuromuscular Junction
Assays
Response Elements
neurons
Synapses
Proteins

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Ecology
  • Molecular Biology
  • Genetics
  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Computational Theory and Mathematics

Cite this

Chen, G., Li, W., Zhang, Q-S., Regulski, M., Sinha, N., Barditch, J., ... Dubnau, J. (2008). Identification of Synaptic Targets of Drosophila Pumilio. PLoS Computational Biology, 4(2), [e1000026]. https://doi.org/10.1371/journal.pcbi.1000026

Identification of Synaptic Targets of Drosophila Pumilio. / Chen, Gengxin; Li, Wanhe; Zhang, Qing-Shuo; Regulski, Michael; Sinha, Nishi; Barditch, Jody; Tully, Tim; Krainer, Adrian R.; Zhang, Michael Q.; Dubnau, Josh.

In: PLoS Computational Biology, Vol. 4, No. 2, e1000026, 02.2008.

Research output: Contribution to journalArticle

Chen, G, Li, W, Zhang, Q-S, Regulski, M, Sinha, N, Barditch, J, Tully, T, Krainer, AR, Zhang, MQ & Dubnau, J 2008, 'Identification of Synaptic Targets of Drosophila Pumilio', PLoS Computational Biology, vol. 4, no. 2, e1000026. https://doi.org/10.1371/journal.pcbi.1000026
Chen G, Li W, Zhang Q-S, Regulski M, Sinha N, Barditch J et al. Identification of Synaptic Targets of Drosophila Pumilio. PLoS Computational Biology. 2008 Feb;4(2). e1000026. https://doi.org/10.1371/journal.pcbi.1000026
Chen, Gengxin ; Li, Wanhe ; Zhang, Qing-Shuo ; Regulski, Michael ; Sinha, Nishi ; Barditch, Jody ; Tully, Tim ; Krainer, Adrian R. ; Zhang, Michael Q. ; Dubnau, Josh. / Identification of Synaptic Targets of Drosophila Pumilio. In: PLoS Computational Biology. 2008 ; Vol. 4, No. 2.
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