Specific high affinity membrane receptor(s) for insulin-like growth factor II have been characterized in two cell lines which produce this hormone and have the ability to proliferate in serum-free media. These receptor(s) have no affinity for either insulin or biosynthetic insulin-like growth factor I. Affinity cross-linking and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed an apparent Mr of 250K which does not change with disulfide bond reduction. Our findings are consistent with an autocrine function for insulin-like growth factor II and indicate that these continuous cell lines may provide unique systems for furthur investigations of this hormone and its receptor.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jan 29 1986|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology