Identification of proteins in human cytomegalovirus (HCMV) particles

The HCMV proteome

Susan M. Varnum, Daniel Streblow, Matthew E. Monroe, Patricia Smith, Kenneth J. Auberry, Ljiljana Paša-Tolić, Dai Wang, David G. Camp, Karin Rodland, Steven Wiley, William Britt, Thomas Shenk, Richard D. Smith, Jay Nelson

Research output: Contribution to journalArticle

395 Citations (Scopus)

Abstract

Human cytomegalovirus (HCMV), a member of the herpesvirus family, is a large complex enveloped virus composed of both viral and cellular gene products. While the sequence of the HCMV genome has been known for over a decade, the fall set of viral and cellular proteins that compose the HCMV virion are unknown. To approach this problem we have utilized gel-free two-dimensional capillary liquid chromatography-tandem mass spectrometry (MS/MS) and Fourier transform ion cyclotron resonance MS to identify and determine the relative abundances of viral and cellular proteins in purified HCMV AD169 virions and dense bodies. Analysis of the proteins from purified HCMV virion preparations has indicated that the particle contains significantly more viral proteins than previously known. In this study, we identified 71 HCMV-encoded proteins that included 12 proteins encoded by known viral open reading frames (ORFs) previously not associated with virions and 12 proteins from novel viral ORFs. Analysis of the relative abundance of HCMV proteins indicated that the predominant virion protein was the pp65 tegument protein and that gM rather than gB was the most abundant glycoprotein. We have also identified over 70 host cellular proteins in HCMV virions, which include cellular structural proteins, enzymes, and chaperones. In addition, analysis of HCMV dense bodies indicated that these viral particles are composed of 29 viral proteins with a reduced quantity of cellular proteins in comparison to HCMV virions. This study provides the first comprehensive quantitative analysis of the viral and cellular proteins that compose infectious particles of a large complex virus.

Original languageEnglish (US)
Pages (from-to)10960-10966
Number of pages7
JournalJournal of Virology
Volume78
Issue number20
DOIs
StatePublished - Oct 2004

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Human herpesvirus 5
Proteome
proteome
Cytomegalovirus
Virion
Viral Proteins
virion
Proteins
proteins
viral proteins
Open Reading Frames
open reading frames
Viruses
Cyclotrons
Herpesviridae
Human Genome
Fourier Analysis
viruses
Tandem Mass Spectrometry
Liquid Chromatography

ASJC Scopus subject areas

  • Immunology

Cite this

Identification of proteins in human cytomegalovirus (HCMV) particles : The HCMV proteome. / Varnum, Susan M.; Streblow, Daniel; Monroe, Matthew E.; Smith, Patricia; Auberry, Kenneth J.; Paša-Tolić, Ljiljana; Wang, Dai; Camp, David G.; Rodland, Karin; Wiley, Steven; Britt, William; Shenk, Thomas; Smith, Richard D.; Nelson, Jay.

In: Journal of Virology, Vol. 78, No. 20, 10.2004, p. 10960-10966.

Research output: Contribution to journalArticle

Varnum, SM, Streblow, D, Monroe, ME, Smith, P, Auberry, KJ, Paša-Tolić, L, Wang, D, Camp, DG, Rodland, K, Wiley, S, Britt, W, Shenk, T, Smith, RD & Nelson, J 2004, 'Identification of proteins in human cytomegalovirus (HCMV) particles: The HCMV proteome', Journal of Virology, vol. 78, no. 20, pp. 10960-10966. https://doi.org/10.1128/JVI.78.20.10960-10966.2004
Varnum, Susan M. ; Streblow, Daniel ; Monroe, Matthew E. ; Smith, Patricia ; Auberry, Kenneth J. ; Paša-Tolić, Ljiljana ; Wang, Dai ; Camp, David G. ; Rodland, Karin ; Wiley, Steven ; Britt, William ; Shenk, Thomas ; Smith, Richard D. ; Nelson, Jay. / Identification of proteins in human cytomegalovirus (HCMV) particles : The HCMV proteome. In: Journal of Virology. 2004 ; Vol. 78, No. 20. pp. 10960-10966.
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