Identification of PN1, a predominant voltage-dependent sodium channel expressed principally in peripheral neurons

Juan J. Toledo-Aral, Brenda L. Moss, Zhi Jun He, Adam G. Koszowski, Teri Whisenand, Simon R. Levinson, John J. Wolf, Inmaculada Silos-Santiago, Simon Halegoua, Gail Mandel

Research output: Contribution to journalArticle

402 Citations (Scopus)

Abstract

Membrane excitability in different tissues is due, in large part, to the selective expression of distinct genes encoding the voltage-dependent sodium channel. Although the predominant sodium channels in brain, skeletal muscle, and cardiac muscle have been identified, the major sodium channel types responsible for excitability within the peripheral nervous system have remained elusive. We now describe the deduced primary structure of a sodium channel, peripheral nerve type 1 (PN1), which is expressed at high levels throughout the peripheral nervous system and is targeted to nerve terminals of cultured dorsal root ganglion neurons. Studies using cultured PC12 cells indicate that both expression and targeting of PN1 is induced by treatment of the cells with nerve growth factor. The preferential localization suggests that the PN1 sodium channel plays a specific role in nerve excitability.

Original languageEnglish (US)
Pages (from-to)1527-1532
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number4
DOIs
StatePublished - Feb 18 1997
Externally publishedYes

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Sodium Channels
Peripheral Nerves
Neurons
Peripheral Nervous System
PC12 Cells
Spinal Ganglia
Nerve Growth Factor
Cultured Cells
Myocardium
Skeletal Muscle
Gene Expression
Membranes
Brain

Keywords

  • dorsal root ganglia
  • nerve growth factor
  • neurites
  • PC12 cells
  • superior cervical ganglia

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Identification of PN1, a predominant voltage-dependent sodium channel expressed principally in peripheral neurons. / Toledo-Aral, Juan J.; Moss, Brenda L.; He, Zhi Jun; Koszowski, Adam G.; Whisenand, Teri; Levinson, Simon R.; Wolf, John J.; Silos-Santiago, Inmaculada; Halegoua, Simon; Mandel, Gail.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 4, 18.02.1997, p. 1527-1532.

Research output: Contribution to journalArticle

Toledo-Aral, JJ, Moss, BL, He, ZJ, Koszowski, AG, Whisenand, T, Levinson, SR, Wolf, JJ, Silos-Santiago, I, Halegoua, S & Mandel, G 1997, 'Identification of PN1, a predominant voltage-dependent sodium channel expressed principally in peripheral neurons', Proceedings of the National Academy of Sciences of the United States of America, vol. 94, no. 4, pp. 1527-1532. https://doi.org/10.1073/pnas.94.4.1527
Toledo-Aral, Juan J. ; Moss, Brenda L. ; He, Zhi Jun ; Koszowski, Adam G. ; Whisenand, Teri ; Levinson, Simon R. ; Wolf, John J. ; Silos-Santiago, Inmaculada ; Halegoua, Simon ; Mandel, Gail. / Identification of PN1, a predominant voltage-dependent sodium channel expressed principally in peripheral neurons. In: Proceedings of the National Academy of Sciences of the United States of America. 1997 ; Vol. 94, No. 4. pp. 1527-1532.
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