Identification of novel regulators of apoptosis using a high-throughput cell-based screen

Kyung Mi Park, Eunju Kang, Yeo Jin Jeon, Nayoung Kim, Nam Soon Kim, Hyang Sook Yoo, Young Il Yeom, Soo Jung Kim

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

High-throughput subcellular imaging is a powerful tool for investigating the function of genes. In order to identify novel regulators of apoptosis we transiently transfected HeLa cells with 938 hypothetical genes of unknown function, and captured their nuclear images with an automated fluorescence microscope. We selected genes that induced greater than 3-fold increase in the percentage of apoptotic nuclei compared with vector-transfected cells. The full-length genes C10orf61, MGC 26717, and FLJ13855 were identified as candidate proapoptotic genes, and their apoptotic effects were confirmed by DNA fragmentation ELISAs and Western blotting for caspase-7 and PARP. We conclude that a subcellular image-based apoptotic screen is useful for identifying genes with proapoptotic activity.

Original languageEnglish (US)
Pages (from-to)170-174
Number of pages5
JournalMolecules and Cells
Volume23
Issue number2
StatePublished - Apr 30 2007

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Keywords

  • Apoptosis
  • Automated fluorescence microscope
  • Hypothetical genes
  • Subcellular imaging

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Park, K. M., Kang, E., Jeon, Y. J., Kim, N., Kim, N. S., Yoo, H. S., Yeom, Y. I., & Kim, S. J. (2007). Identification of novel regulators of apoptosis using a high-throughput cell-based screen. Molecules and Cells, 23(2), 170-174.