Identification of Insulin-Like Growth Factor-Binding Protein-3 (IGFBP-3) and IGFBP-2 in Human Follicular Fluid

Linda C. Giudice, Eleanor M. Farrell, Hung Pham, Ron G. Rosenfeld

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Two major insulin-like growth factor-binding proteins, IGFBP-2 and IGFBP-3, were identified by Western ligand blotting of human follicular fluid (FF) aspirated from luteinizing follicles during ovarian stimulation with human menopausal gonadotropin (LH and FSH). IGFBP-1 was also present in FF, and an IGFBP with a mol wt of 24000 daltons, present in serum, appeared to be absent in FF. Identification of IGFBP-1, IGFBP-2, and IGFBP-3 was confirmed using IGFBP-specific antisera. IGF-I and IGF-II levels were measured in patient serum and FF at the time of oocyte harvest, and serum levels of both peptides were consistently higher than FF levels. The finding of three major serum IGFBPs in human FF suggests that IGF regulation in the ovary is probably under complex control by this set of regulatory BPs.

Original languageEnglish (US)
Pages (from-to)1330-1338
Number of pages9
JournalJournal of Clinical Endocrinology and Metabolism
Volume71
Issue number5
DOIs
StatePublished - Nov 1990

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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