Identification of insulin-like growth factor-binding protein-3 (IGFBP-3) and IGFBP-2 in human follicular fluid

Linda C. Giudice, Eleanor M. Farrell, Hung Pham, Ronald (Ron) Rosenfeld

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Two major insulin-like growth factor-binding proteins, IGFBP-2 and IGFBP-3, were identified by Western ligand blotting of human follicular fluid (FF) aspirated from luteinizing follicles during ovarian stimulation with human menopausal gonadotropin (LH and FSH). IGFBP-1 was also present in FF, and an IGFBP with a mol wt of 24000 daltons, present in serum, appeared to be absent in FF. Identification of IGFBP-1, IGFBP-2, and IGFBP-3 was confirmed using IGFBP-specific antisera. IGF-I and IGF-II levels were measured in patient serum and FF at the time of oocyte harvest, and serum levels of both peptides were consistently higher than FF levels. The finding of three major serum IGFBPs in human FF suggests that IGF regulation in the ovary is probably under complex control by this set of regulatory BPs.

Original languageEnglish (US)
Pages (from-to)1330-1338
Number of pages9
JournalJournal of Clinical Endocrinology and Metabolism
Volume71
Issue number5
StatePublished - Nov 1990
Externally publishedYes

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Insulin-Like Growth Factor Binding Protein 2
Follicular Fluid
Insulin-Like Growth Factor Binding Protein 3
Insulin-Like Growth Factor Binding Proteins
Fluids
Insulin-Like Growth Factor Binding Protein 1
Serum
Menotropins
Insulin-Like Growth Factor II
Ovulation Induction
Insulin-Like Growth Factor I
Oocytes
Immune Sera
Ovary
Western Blotting
Ligands
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Identification of insulin-like growth factor-binding protein-3 (IGFBP-3) and IGFBP-2 in human follicular fluid. / Giudice, Linda C.; Farrell, Eleanor M.; Pham, Hung; Rosenfeld, Ronald (Ron).

In: Journal of Clinical Endocrinology and Metabolism, Vol. 71, No. 5, 11.1990, p. 1330-1338.

Research output: Contribution to journalArticle

Giudice, Linda C. ; Farrell, Eleanor M. ; Pham, Hung ; Rosenfeld, Ronald (Ron). / Identification of insulin-like growth factor-binding protein-3 (IGFBP-3) and IGFBP-2 in human follicular fluid. In: Journal of Clinical Endocrinology and Metabolism. 1990 ; Vol. 71, No. 5. pp. 1330-1338.
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