Identification of genes associated with mutacin I production in Streptococcus mutans using random insertional mutagenesis

Phoebe Tsang, Justin Merritt, Trang Nguyen, Wenyuan Shi, Fengxia Qi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Streptococcus mutans is a major pathogen implicated in dental caries. Its virulence is enhanced by its ability to produce bacteriocins, called mutacins, which inhibit the growth of other Gram-positive bacteria. The goal of this study is to use a random insertional mutagenesis approach to search for genes that are associated with mutacin I production in the virulent strain UA140. A random insertional mutagenesis library consisting of 11 000 clones was constructed and screened for a mutacin-defective phenotype. Mutacin-defective clones were isolated, and their insertion sites were determined by PCR amplification or plasmid rescue followed by sequencing. A total of twenty-five unique genes were identified. These genes can be categorized into the following functional classes: two-component sensory systems, stress responses, energy metabolism and central cellular processes. Several conserved hypothetical proteins with unknown functions were also identified. These results suggest that mutacin I production is stringently controlled by diverse and complex regulatory pathways.

Original languageEnglish (US)
Pages (from-to)3947-3955
Number of pages9
JournalMicrobiology
Volume151
Issue number12
DOIs
StatePublished - Dec 2005
Externally publishedYes

Fingerprint

Streptococcus mutans
Insertional Mutagenesis
Clone Cells
Genes
Bacteriocins
Dental Caries
Gram-Positive Bacteria
Energy Metabolism
Libraries
Virulence
Plasmids
Phenotype
Polymerase Chain Reaction
Growth
mutacin I
Proteins

ASJC Scopus subject areas

  • Microbiology

Cite this

Identification of genes associated with mutacin I production in Streptococcus mutans using random insertional mutagenesis. / Tsang, Phoebe; Merritt, Justin; Nguyen, Trang; Shi, Wenyuan; Qi, Fengxia.

In: Microbiology, Vol. 151, No. 12, 12.2005, p. 3947-3955.

Research output: Contribution to journalArticle

Tsang, Phoebe ; Merritt, Justin ; Nguyen, Trang ; Shi, Wenyuan ; Qi, Fengxia. / Identification of genes associated with mutacin I production in Streptococcus mutans using random insertional mutagenesis. In: Microbiology. 2005 ; Vol. 151, No. 12. pp. 3947-3955.
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