TY - JOUR
T1 - Identification of differentially expressed genes in human lung squamous cell carcinoma using suppression subtractive hybridization
AU - Sun, Wenyue
AU - Zhang, Kaitai
AU - Zhang, Xinyu
AU - Lei, Wendong
AU - Xiao, Ting
AU - Ma, Jinfang
AU - Guo, Suping
AU - Shao, Shujuan
AU - Zhang, Husheng
AU - Liu, Yan
AU - Yuan, Jinsong
AU - Hu, Zhi
AU - Ma, Ying
AU - Feng, Xiaoli
AU - Hu, Songnian
AU - Zhou, Jun
AU - Cheng, Shujun
AU - Gao, Yanning
N1 - Funding Information:
This work was supported by the State Key Programme of Basic Research (Project No. G1998051207), the National Key Technologies R&D Programme (Project No. 2002BA711A06) from the Ministry of Science and Technology, P.R. China, and grant of the National Natural Science Foundation of China (Project No. 30070836).
PY - 2004/8/20
Y1 - 2004/8/20
N2 - Lung cancer is one of the major causes of cancer-related deaths. Over the past decade, much has been known about the molecular changes associated with lung carcinogenesis; however, our understanding to lung tumorigenesis is still incomplete. To identify genes that are differentially expressed in squamous cell carcinoma (SCC) of the lung, we compared the expression profiles between primarily cultured SCC tumor cells and bronchial epithelial cells derived from morphologically normal bronchial epithelium of the same patient. Using suppression subtractive hybridization (SSH), two cDNA libraries containing up- and down-regulated genes in the tumor cells were constructed, named as LCTP and LCBP. The two libraries comprise 258 known genes and 133 unknown genes in total. The known up-regulated genes in the library LCTP represented a variety of functional groups; including metabolism-, cell adhesion and migration-, signal transduction-, and anti-apoptosis-related genes. Using semi-quantitative reverse transcription-polymerase chain reaction, seven genes chosen randomly from the LCTP were analyzed in the tumor tissue paired with its corresponding adjacent normal lung tissue derived from 16 cases of the SCC. Among them, the IQGAP1, RAP1GDS1, PAICS, MLF1, and MARK1 genes showed a consistent expression pattern with that of the SSH analysis. Identification and further characterization of these genes may allow a better understanding of lung carcinogenesis.
AB - Lung cancer is one of the major causes of cancer-related deaths. Over the past decade, much has been known about the molecular changes associated with lung carcinogenesis; however, our understanding to lung tumorigenesis is still incomplete. To identify genes that are differentially expressed in squamous cell carcinoma (SCC) of the lung, we compared the expression profiles between primarily cultured SCC tumor cells and bronchial epithelial cells derived from morphologically normal bronchial epithelium of the same patient. Using suppression subtractive hybridization (SSH), two cDNA libraries containing up- and down-regulated genes in the tumor cells were constructed, named as LCTP and LCBP. The two libraries comprise 258 known genes and 133 unknown genes in total. The known up-regulated genes in the library LCTP represented a variety of functional groups; including metabolism-, cell adhesion and migration-, signal transduction-, and anti-apoptosis-related genes. Using semi-quantitative reverse transcription-polymerase chain reaction, seven genes chosen randomly from the LCTP were analyzed in the tumor tissue paired with its corresponding adjacent normal lung tissue derived from 16 cases of the SCC. Among them, the IQGAP1, RAP1GDS1, PAICS, MLF1, and MARK1 genes showed a consistent expression pattern with that of the SSH analysis. Identification and further characterization of these genes may allow a better understanding of lung carcinogenesis.
KW - Differential expression
KW - Squamous cell carcinoma
KW - Suppression subtractive hybridization
UR - http://www.scopus.com/inward/record.url?scp=3042858956&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042858956&partnerID=8YFLogxK
U2 - 10.1016/j.canlet.2004.03.023
DO - 10.1016/j.canlet.2004.03.023
M3 - Article
C2 - 15246564
AN - SCOPUS:3042858956
SN - 0304-3835
VL - 212
SP - 83
EP - 93
JO - Cancer Letters
JF - Cancer Letters
IS - 1
ER -