Identification of cytomegalovirus in a liquid-based gynecologic sample using morphology, immunohistochemistry, and DNA real-time PCR detection

Harmanjatinder S. Sekhon, Richard Press, Waldemar A. Schmidt, Mary Hawley, Anne Rader

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The viral cytopathic effects of cytomegalovirus (CMV) are rarely encountered in conventional cervical vaginal smears and have never been reported in a liquid-based gynecologic sample (Sure-Path). We present results of a liquid-based gynecologic sample (SurePath) from an asymptomatic patient with classic CMV-associated granular or dense cyanophilic intracytoplasmic and intranuclear inclusion bodies with a clear surrounding zone. These inclusions were immunohistochemically positive for CMV. The patient also had human papilloma virus (HPV)-associated koilocytes that showed a unique perinuclear immunostaining pattern suggesting coinfection with both CMV and HPV. CMV amplification using real-time polymerase chain reaction (PCR) of DNA extracted from the liquid-based sample confirmed the morphologic and immunohistochemical findings of CMV infection. These observations suggest that a liquid-based preparation can be used to assess CMV infection morphologically, immunohistochemically, and by real-time PCR.

Original languageEnglish (US)
Pages (from-to)411-417
Number of pages7
JournalDiagnostic Cytopathology
Volume30
Issue number6
DOIs
StatePublished - Jun 2004

Fingerprint

Cytomegalovirus
Real-Time Polymerase Chain Reaction
Papillomaviridae
Immunohistochemistry
DNA
Vaginal Smears
Cytomegalovirus Infections
Viral Cytopathogenic Effect
Intranuclear Inclusion Bodies
Coinfection

Keywords

  • Cervicitis
  • Cytomegalovirus
  • Liquid-based cytology
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Anatomy

Cite this

Identification of cytomegalovirus in a liquid-based gynecologic sample using morphology, immunohistochemistry, and DNA real-time PCR detection. / Sekhon, Harmanjatinder S.; Press, Richard; Schmidt, Waldemar A.; Hawley, Mary; Rader, Anne.

In: Diagnostic Cytopathology, Vol. 30, No. 6, 06.2004, p. 411-417.

Research output: Contribution to journalArticle

Sekhon, Harmanjatinder S. ; Press, Richard ; Schmidt, Waldemar A. ; Hawley, Mary ; Rader, Anne. / Identification of cytomegalovirus in a liquid-based gynecologic sample using morphology, immunohistochemistry, and DNA real-time PCR detection. In: Diagnostic Cytopathology. 2004 ; Vol. 30, No. 6. pp. 411-417.
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