Identification of cDNA clones of the mouse neural cell adhesion molecule L1

Roland Tacke, Marion Moos, David B. Teplow, Klaus Frueh, Herta Scherer, Alfred Bach, Melitta Schachner

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Two cDNA clones of the neural cell adhesion molecule L1 (Mr 200,000) were isolated using λgt10 and λgt11 libraries constructed from postnatal day 8 mouse brain poly(A)+ RNA. Clone K21 was selected and identified using immunoaffinity purified polyclonal antibodies. It was then used to isolate a secondary clone (K21-1), which hybridized with an oligonucleotide probe synthesized by reverse translation of the aminoterminal sequence of the 80 kDa carboxyterminal proteolytic fragment of L1. Blot hybridization analysis indicated that L1 is encoded by a single gene and transcribed by a single 6 kb mRNA which is present only in cells or tissues known to express L1.

Original languageEnglish (US)
Pages (from-to)89-94
Number of pages6
JournalNeuroscience Letters
Volume82
Issue number1
DOIs
StatePublished - Nov 10 1987
Externally publishedYes

Fingerprint

Neural Cell Adhesion Molecule L1
Complementary DNA
Clone Cells
Messenger RNA
Oligonucleotide Probes
Libraries
Antibodies
Brain
Genes

Keywords

  • cDNA
  • Cell adhesion
  • L1
  • Mouse
  • Nervous system

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Tacke, R., Moos, M., Teplow, D. B., Frueh, K., Scherer, H., Bach, A., & Schachner, M. (1987). Identification of cDNA clones of the mouse neural cell adhesion molecule L1. Neuroscience Letters, 82(1), 89-94. https://doi.org/10.1016/0304-3940(87)90176-5

Identification of cDNA clones of the mouse neural cell adhesion molecule L1. / Tacke, Roland; Moos, Marion; Teplow, David B.; Frueh, Klaus; Scherer, Herta; Bach, Alfred; Schachner, Melitta.

In: Neuroscience Letters, Vol. 82, No. 1, 10.11.1987, p. 89-94.

Research output: Contribution to journalArticle

Tacke, R, Moos, M, Teplow, DB, Frueh, K, Scherer, H, Bach, A & Schachner, M 1987, 'Identification of cDNA clones of the mouse neural cell adhesion molecule L1', Neuroscience Letters, vol. 82, no. 1, pp. 89-94. https://doi.org/10.1016/0304-3940(87)90176-5
Tacke, Roland ; Moos, Marion ; Teplow, David B. ; Frueh, Klaus ; Scherer, Herta ; Bach, Alfred ; Schachner, Melitta. / Identification of cDNA clones of the mouse neural cell adhesion molecule L1. In: Neuroscience Letters. 1987 ; Vol. 82, No. 1. pp. 89-94.
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