Identification of an IQGAP1/AKAP79 complex in β-cells

J. Brian Nauert, Johanna D. Rigas, Linda B. Lester

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

IQGAP1, is a recently discovered scaffold protein proposed to regulate membrane cytoskeleton events through protein-protein interactions with F-actin, E-cadherin, β-catenin, and CLIP170. The binding of IQGAP1 to its partners is regulated by calcium/calmodulin (Ca++/CaM) and the small molecular weight guanine nucleotide triphosphatases (GTPases), Cdc42, and Rac1. Here we identify a novel IQGAP1 scaffolding function by isolating the cyclic AMP dependent kinase (PKA) with IQGAP1. IQGAP1 was co-purified with PKA using 5′-cyclic AMP (cAMP) affinity chromatography and PKA activity was co-immunoprecipitated with IQGAP1 using an anti-IQGAP1 antibody. The association of IQGAP1 with PKA was shown to occur through a direct interaction between A kinase anchoring protein 79 (AKAP79) and the carboxyl-terminal domain of IQGAP1. This suggests that cAMP/PKA may be coupled with Ca++/CaM and GTPases through an IQGAP1/AKAP79 complex.

Original languageEnglish (US)
Pages (from-to)97-108
Number of pages12
JournalJournal of cellular biochemistry
Volume90
Issue number1
DOIs
StatePublished - Sep 1 2003

Keywords

  • β-cells
  • CAMP/PKA
  • GTPases
  • IQGAP
  • Rho family
  • Signal transduct

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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