Abstract
IQGAP1, is a recently discovered scaffold protein proposed to regulate membrane cytoskeleton events through protein-protein interactions with F-actin, E-cadherin, β-catenin, and CLIP170. The binding of IQGAP1 to its partners is regulated by calcium/calmodulin (Ca++/CaM) and the small molecular weight guanine nucleotide triphosphatases (GTPases), Cdc42, and Rac1. Here we identify a novel IQGAP1 scaffolding function by isolating the cyclic AMP dependent kinase (PKA) with IQGAP1. IQGAP1 was co-purified with PKA using 5′-cyclic AMP (cAMP) affinity chromatography and PKA activity was co-immunoprecipitated with IQGAP1 using an anti-IQGAP1 antibody. The association of IQGAP1 with PKA was shown to occur through a direct interaction between A kinase anchoring protein 79 (AKAP79) and the carboxyl-terminal domain of IQGAP1. This suggests that cAMP/PKA may be coupled with Ca++/CaM and GTPases through an IQGAP1/AKAP79 complex.
Original language | English (US) |
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Pages (from-to) | 97-108 |
Number of pages | 12 |
Journal | Journal of cellular biochemistry |
Volume | 90 |
Issue number | 1 |
DOIs | |
State | Published - Sep 1 2003 |
Keywords
- CAMP/PKA
- GTPases
- IQGAP
- Rho family
- Signal transduct
- β-cells
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology