Identification of an immunodominant cytotoxic T-lymphocyte recognition site in glycoprotein B of herpes simplex virus by using recombinant adenovirus vectors and synthetic peptides

T. Hanke, F. L. Graham, K. L. Rosenthal, D. C. Johnson

Research output: Contribution to journalArticle

93 Scopus citations

Abstract

Cytotoxic T-lymphocyte (CTL) responses to herpes simplex virus (HSV) polypeptides play an important role in recovery from infection and in preventing latency. We have previously shown that glycoprotein B (gB) is a major target recognized by HSV-specific CTLs in C57BL/6 (H-2(b)) and BALB/c (H-2(d)) mice but not in CBA/J (H-2(k)) mice (L. A. Witmer, K. L. Rosenthal, F. L. Graham, H. M. Friedman, A. Yee, and D. C. Johnson, J. Gen. Virol. 71:387-396, 1990). In this report, we utilize adenovirus vectors expressing gB with various deletions to localize an immunodominant site in gB, recognized by H-2(b)-restricted anti-HSV CTLs, to a region between residues 462 and 594. Overlapping peptides spanning this region were synthesized and used to further localize the immunodominant site to residues 489 to 515, a region highly conserved in HSV type 1 (HSV-1) and HSV-2 strains. The 11-amino-acid peptide was apparently associated exclusively with the K(b) major histocompatibility complex gene product and not the D(b) gene product. In contrast, H-2(d)-restricted CTLs recognized an immunodominant site between residues 233 and 379.

Original languageEnglish (US)
Pages (from-to)1177-1186
Number of pages10
JournalJournal of virology
Volume65
Issue number3
StatePublished - Mar 20 1991

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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