Identification of an epidermal growth factor-related transforming growth factor from rat fetuses

Lynn M. Matrisian; Meere Pathak; Bruce E. Magun

doi:10.1016/0006-291X(82)90589-7

Identification of an epidermal growth factor-related transforming growth factor from rat fetuses

Lynn M. Matrisian, Meere Pathak, Bruce E. Magun

Research output: Contribution to journal › Article › peer-review

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Abstract

An epidermal growth factor (EGF)-like transforming growth factor was identified in acid/ethanol extracts of 19-day-old rat embryos. This factor had an apparent molecular weight of 55,000 and possessed EGF-competing activity, stimulated DNA synthesis in quiescent Rat-1 fibroblasts, and induced progressive growth of untransformed cells in soft agar. The factor was trypsin sensitive and required intact disulfide bonds for colony stimulating activity. It was determined to be distinct from adult mouse submaxillary gland EGF based on its elution from BioGel P-60 column and its increased colony stimulating activity/unit of EGF-competing activity.

Original language	English (US)
Pages (from-to)	761-769
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	107
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(82)90589-7
State	Published - Aug 16 1982
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(82)90589-7

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abstract = "An epidermal growth factor (EGF)-like transforming growth factor was identified in acid/ethanol extracts of 19-day-old rat embryos. This factor had an apparent molecular weight of 55,000 and possessed EGF-competing activity, stimulated DNA synthesis in quiescent Rat-1 fibroblasts, and induced progressive growth of untransformed cells in soft agar. The factor was trypsin sensitive and required intact disulfide bonds for colony stimulating activity. It was determined to be distinct from adult mouse submaxillary gland EGF based on its elution from BioGel P-60 column and its increased colony stimulating activity/unit of EGF-competing activity.",

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AU - Magun, Bruce E.

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Y1 - 1982/8/16

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AB - An epidermal growth factor (EGF)-like transforming growth factor was identified in acid/ethanol extracts of 19-day-old rat embryos. This factor had an apparent molecular weight of 55,000 and possessed EGF-competing activity, stimulated DNA synthesis in quiescent Rat-1 fibroblasts, and induced progressive growth of untransformed cells in soft agar. The factor was trypsin sensitive and required intact disulfide bonds for colony stimulating activity. It was determined to be distinct from adult mouse submaxillary gland EGF based on its elution from BioGel P-60 column and its increased colony stimulating activity/unit of EGF-competing activity.

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Identification of an epidermal growth factor-related transforming growth factor from rat fetuses

Abstract

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