Identification of an epidermal growth factor-related transforming growth factor from rat fetuses

Lynn M. Matrisian, Meere Pathak, Bruce E. Magun

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

An epidermal growth factor (EGF)-like transforming growth factor was identified in acid/ethanol extracts of 19-day-old rat embryos. This factor had an apparent molecular weight of 55,000 and possessed EGF-competing activity, stimulated DNA synthesis in quiescent Rat-1 fibroblasts, and induced progressive growth of untransformed cells in soft agar. The factor was trypsin sensitive and required intact disulfide bonds for colony stimulating activity. It was determined to be distinct from adult mouse submaxillary gland EGF based on its elution from BioGel P-60 column and its increased colony stimulating activity/unit of EGF-competing activity.

Original languageEnglish (US)
Pages (from-to)761-769
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume107
Issue number3
DOIs
StatePublished - Aug 16 1982
Externally publishedYes

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Transforming Growth Factor alpha
Epidermal Growth Factor
Rats
Fetus
Submandibular Gland
Transforming Growth Factors
Fibroblasts
Disulfides
Trypsin
Agar
Ethanol
Embryonic Structures
Molecular Weight
Molecular weight
Cells
Acids
DNA
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of an epidermal growth factor-related transforming growth factor from rat fetuses. / Matrisian, Lynn M.; Pathak, Meere; Magun, Bruce E.

In: Biochemical and Biophysical Research Communications, Vol. 107, No. 3, 16.08.1982, p. 761-769.

Research output: Contribution to journalArticle

Matrisian, Lynn M. ; Pathak, Meere ; Magun, Bruce E. / Identification of an epidermal growth factor-related transforming growth factor from rat fetuses. In: Biochemical and Biophysical Research Communications. 1982 ; Vol. 107, No. 3. pp. 761-769.
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