Identification of an epidermal growth factor-related transforming growth factor from rat fetuses

Lynn M. Matrisian, Meere Pathak, Bruce E. Magun

    Research output: Contribution to journalArticlepeer-review

    43 Scopus citations

    Abstract

    An epidermal growth factor (EGF)-like transforming growth factor was identified in acid/ethanol extracts of 19-day-old rat embryos. This factor had an apparent molecular weight of 55,000 and possessed EGF-competing activity, stimulated DNA synthesis in quiescent Rat-1 fibroblasts, and induced progressive growth of untransformed cells in soft agar. The factor was trypsin sensitive and required intact disulfide bonds for colony stimulating activity. It was determined to be distinct from adult mouse submaxillary gland EGF based on its elution from BioGel P-60 column and its increased colony stimulating activity/unit of EGF-competing activity.

    Original languageEnglish (US)
    Pages (from-to)761-769
    Number of pages9
    JournalBiochemical and Biophysical Research Communications
    Volume107
    Issue number3
    DOIs
    StatePublished - Aug 16 1982

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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