Identification of amino acid residues required for ferric-anguibactin transport in the outer-membrane receptor FatA of Vibrio anguillarum

Claudia Lopez, Alejandro F. Alice, Ranjan Chakraborty, Jorge H. Crosa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Vibrio anguillarum 775 is a fish pathogen that causes a disease characterized by a fatal haemorrhagic septicaemia. It harbours the 65 kbp pJM1 plasmid, which encodes an iron sequestering system specific for the siderophore anguibactin and is essential for virulence. The genes involved in the biosynthesis of anguibactin are located on both the pJM1 plasmid and the chromosome. However, the genes for the outer-membrane receptor FatA and the other transport proteins are only carried on the plasmid. With the aim of elucidating the mechanism of ferric-anguibactin transport mediated by FatA, this work focuses on the identification of FatA amino acid residues that play a role in the transport of ferric-anguibactin, by analysing the transport kinetics of site-directed mutants. The mutations studied were located in conserved residues of the lock region, which contains a cluster of ten residues belonging to the N-terminal and barrel domains, and of the channel region of FatA, which contains conserved glycines located in the β5-β6 loop and a conserved arginine located in strand 11 of the β-barrel. In the case of the FatA lock region, it is clear that although the residues analysed in this work (R95, K130, E505 and E550) are conserved among various outer-membrane receptors, their involvement in the transport process might differ among receptors. Furthermore, it was determined that in the FatA channel region double substitutions of the conserved glycines 131 and 143 with alanine resulted in a variant receptor unable to transport ferric-anguibactin. It was also shown that the conserved arginine 428 located in strand 11 is essential for transport. The results suggest that a conformational change or partial unfolding of the plug domain occurs during ferric-anguibactin transport.

Original languageEnglish (US)
Pages (from-to)570-584
Number of pages15
JournalMicrobiology
Volume153
Issue number2
DOIs
StatePublished - Feb 2007

Fingerprint

Vibrio
Amino Acids
Membranes
Plasmids
Glycine
Arginine
Hemorrhagic Septicemia
Siderophores
Alanine
Genes
Virulence
anguibactin
Carrier Proteins
Fishes
Iron
Chromosomes
Mutation

ASJC Scopus subject areas

  • Microbiology

Cite this

Identification of amino acid residues required for ferric-anguibactin transport in the outer-membrane receptor FatA of Vibrio anguillarum. / Lopez, Claudia; Alice, Alejandro F.; Chakraborty, Ranjan; Crosa, Jorge H.

In: Microbiology, Vol. 153, No. 2, 02.2007, p. 570-584.

Research output: Contribution to journalArticle

Lopez, Claudia ; Alice, Alejandro F. ; Chakraborty, Ranjan ; Crosa, Jorge H. / Identification of amino acid residues required for ferric-anguibactin transport in the outer-membrane receptor FatA of Vibrio anguillarum. In: Microbiology. 2007 ; Vol. 153, No. 2. pp. 570-584.
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