Identification of a short sequence in the HCMV terminase pUL56 essential for interaction with pUL89 subunit

G. Ligat, C. Jacquet, Sunwen Chou, A. Couvreux, S. Alain, S. Hantz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The human cytomegalovirus (HCMV) terminase complex consists of several components acting together to cleave viral DNA into unit length genomes and translocate them into capsids, a critical process in the production of infectious virions subsequent to DNA replication. Previous studies suggest that the carboxyl-terminal portion of the pUL56 subunit interacts with the pUL89 subunit. However, the specific interacting residues of pUL56 remain unknown. We identified a conserved sequence in the C-terminal moiety of pUL56 (671WMVVKYMGFF680). Overrepresentation of conserved aromatic amino acids through 20 herpesviruses homologues of pUL56 suggests an involvement of this short peptide into the interaction between the larger pUL56 terminase subunit and the smaller pUL89 subunit. Use of Alpha technology highlighted an interaction between pUL56 and pUL89 driven through the peptide 671WMVVKYMGFF680. A deletion of these residues blocks viral replication. We hypothesize that it is the consequence of the disruption of the pUL56-pUL89 interaction. These results show that this motif is essential for HCMV replication and could be a target for development of new small antiviral drugs or peptidomimetics.

Original languageEnglish (US)
Article number8796
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Cytomegalovirus
Peptidomimetics
Aromatic Amino Acids
Peptides
Conserved Sequence
Capsid
Herpesviridae
Viral DNA
DNA Replication
Virion
Antiviral Agents
Genome
Technology
terminase

ASJC Scopus subject areas

  • General

Cite this

Identification of a short sequence in the HCMV terminase pUL56 essential for interaction with pUL89 subunit. / Ligat, G.; Jacquet, C.; Chou, Sunwen; Couvreux, A.; Alain, S.; Hantz, S.

In: Scientific Reports, Vol. 7, No. 1, 8796, 01.12.2017.

Research output: Contribution to journalArticle

Ligat, G. ; Jacquet, C. ; Chou, Sunwen ; Couvreux, A. ; Alain, S. ; Hantz, S. / Identification of a short sequence in the HCMV terminase pUL56 essential for interaction with pUL89 subunit. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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