Identification of a novel herpes simplex virus type 1-induced glycoprotein which complexes with gE and binds immunoglobulin

David Johnson, V. Feenstra

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

We detected a glycoprotein on the surface of cells infected with herpes simplex virus type 1 (HSV-1) which, in conjuction with gE, binds immunoglobulin G (IgG). The novel glycoprotein, which has an apparent molecular mass of 70 kilodaltons and was provisionally named g70, was first detected in extracts of HSV-1-infected cells labeled by lactoperoxidase-catalyzed iodination and precipitated with rabbit sera or IgG and protein A-Sepharose. In subsequent experiments, g70 and gE were coprecipitated from extracts of HSV-1-infected cells labeled with [35S]methionine, [35S]cysteine, or 14C-amino acids. We were unable to precipitate a polypeptide analogous to g70 or gE from extracts of HSV-2-infected cells with rabbit IgG and protein A-Sepharose. Partial proteolytic peptide analysis indicated that g70 is structurally distinct from gE and gD. In addition, g70 was electrophoretically distinct from the HSV-1 Us4 glycoprotein gG. HSV-1 gE, expressed in mouse cells transfected with the gE gene, was not precipitated with rabbit IgG, nor could these cells bind radiolabeled IgG, suggesting that gE alone cannot act as an IgG (Fc) receptor. This result, coupled with the findings that gE and g70 are coprecipitated with IgG and with an anti-gE monoclonal antibody, suggests that gE and g70 form a complex which binds IgG. The electrophoretic mobilities of g70 molecules induced by different strains of HSV-1 differed markedly, arguing that g70 is encoded by the virus and is not a cellular protein induced by virus infection.

Original languageEnglish (US)
Pages (from-to)2208-2216
Number of pages9
JournalJournal of Virology
Volume61
Issue number7
StatePublished - 1987
Externally publishedYes

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Human herpesvirus 1
immunoglobulin G
Human Herpesvirus 1
immunoglobulins
Immunoglobulins
glycoproteins
Glycoproteins
Immunoglobulin G
Rabbits
rabbits
GTP-Binding Proteins
cells
agarose
extracts
Lactoperoxidase
IgG Receptors
iodination
Peptides
Human Herpesvirus 2
Fc Receptors

ASJC Scopus subject areas

  • Immunology

Cite this

Identification of a novel herpes simplex virus type 1-induced glycoprotein which complexes with gE and binds immunoglobulin. / Johnson, David; Feenstra, V.

In: Journal of Virology, Vol. 61, No. 7, 1987, p. 2208-2216.

Research output: Contribution to journalArticle

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