Identification of a novel guanylyl cyclase that is related to receptor guanylyl cyclases, but lacks extracellular and transmembrane domains

P. Jeanette Simpson, Alan Nighorn, David Morton

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We have identified a novel guanylyl cyclase, named MsGC-I, that is expressed in the nervous system of Manduca sexta. MsGC-I shows highest sequence identity with receptor guanylyl cyclases throughout its catalytic and dimerization domains but does not contain the ligand-binding, transmembrane, or kinase-like domains characteristic of receptor guanylyl cyclases. In addition, MsGC-I contains a C-terminal extension of 149 amino acids that is not present in other receptor guanylyl cyclases. The sequence of MsGC-I contains no regions that show similarity to the regulatory domain of soluble guanylyl cyclases. Thus, MsGC-I appears to represent a member of a new class of guanylyl cyclases. We show that both a transcript and a protein of the sizes predicted from the MsGC-I cDNA are present in the nervous system of Manduca and that MsGC-I is expressed in a small population of neurons within the abdominal ganglia. When expressed in COS-7 cells, MsGC-I appears to exist as a soluble homodimer with high levels of basal guanylyl cyclase activity that is insensitive to stimulation by nitric oxide. Western blot analysis, however, shows that MsGC-I is localized to the particulate fraction of nervous system homogenates, suggesting that it may be membrane-associated in vivo.

Original languageEnglish (US)
Pages (from-to)4440-4446
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number7
DOIs
StatePublished - Feb 12 1999

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Guanylate Cyclase-Coupled Receptors
Guanylate Cyclase
Nervous System
Manduca
Neurology
COS Cells
Dimerization
Ganglia
Catalytic Domain
Nitric Oxide
Phosphotransferases
Complementary DNA
Western Blotting
Neurons
Ligands
Amino Acids
Membranes
Population
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Identification of a novel guanylyl cyclase that is related to receptor guanylyl cyclases, but lacks extracellular and transmembrane domains. / Simpson, P. Jeanette; Nighorn, Alan; Morton, David.

In: Journal of Biological Chemistry, Vol. 274, No. 7, 12.02.1999, p. 4440-4446.

Research output: Contribution to journalArticle

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