Identification of a human liver cytochrome P-450 exhibiting catalytic and immunochemical similarities to cytochrome P-450 3a of rabbit liver

Judy Raucy, Peter Fernandes, Martin Black, Shin L. Yang, Dennis Koop

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Abstract

Immunoblot analysis of liver microsomes from nine patients demonstrated that each contained a cytochrome P-450 that reacted with an antibody directed against the ethanol-inducible rabbit liver cytochrome, P-450 3a. Two of the liver specimens exhibited high concentrations of the immunoreactive protein, high rates of aniline hydroxylation and N-nitrosodimethylamine demethylation, and extensive inhibition of activity in the presence of antibody to P-450 3a. One other liver specimen exhibited a very low rate of aniline hydroxylation with significantly less antibody inhibition. The variability witnessed was independent of the alcohol history of the individual patients, suggesting that the human cytochrome may be under some other environmental, dietary or genetic regulation. Its inducibility by ethanol was not directly studied in this investigation. However, we conclude that there is a cytochrome P-450 present in human liver which is immunochemically and catalytically similar to the ethanol-inducible P-450 of rabbit liver.

Original languageEnglish (US)
Pages (from-to)2921-2926
Number of pages6
JournalBiochemical Pharmacology
Volume36
Issue number18
DOIs
StatePublished - Sep 15 1987
Externally publishedYes

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Forensic Anthropology
Liver
Cytochrome P-450 Enzyme System
Rabbits
Ethanol
Hydroxylation
Antibodies
Dimethylnitrosamine
Liver Microsomes
Cytochromes
Alcohols
Proteins

ASJC Scopus subject areas

  • Pharmacology

Cite this

Identification of a human liver cytochrome P-450 exhibiting catalytic and immunochemical similarities to cytochrome P-450 3a of rabbit liver. / Raucy, Judy; Fernandes, Peter; Black, Martin; Yang, Shin L.; Koop, Dennis.

In: Biochemical Pharmacology, Vol. 36, No. 18, 15.09.1987, p. 2921-2926.

Research output: Contribution to journalArticle

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