Identification of a developmentally regulated gene, esr16, in the tracheal epithelium of Manduca sexta, with homology to a protein from human epididymis

Melinda Mészáros, David Morton

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Events related to both ecdysis and metamorphosis require the expression of a new set of genes, the majority of which are regulated by the changes in ecdysteroid levels. We have initiated studies to identify genes whose expression is up-regulated between 24 and 4 h before pupal ecdysis in Manduca sexta. In this paper we report the partial characterization of one such gene, esr16. The transcript of esr16 is detected by Northern blot analysis in nervous tissue, muscle and trachea isolated from animals 4 h before, but not 24 h before pupal ecdysis. In situ hybridization showed that the transcript was expressed in epithelial cells of the large tracheae surrounding the nervous system and muscle. Sequence analysis suggested that the gene encoded a secreted protein with 35% identity to HE1, a human epididymal-specific gene.

Original languageEnglish (US)
Pages (from-to)7-11
Number of pages5
JournalInsect Biochemistry and Molecular Biology
Volume26
Issue number1
StatePublished - 1996
Externally publishedYes

Fingerprint

Manduca
Epididymis
epididymis
Manduca sexta
Molting
ecdysis
epithelium
Epithelium
Genes
trachea (vertebrates)
Trachea
Muscle
Proteins
genes
proteins
Ecdysteroids
Nerve Tissue
Muscles
ecdysteroids
Neurology

Keywords

  • Ecdysone
  • Ecdysteroid
  • Epididymis
  • Gene regulation
  • Manduca
  • Tracheae

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry

Cite this

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abstract = "Events related to both ecdysis and metamorphosis require the expression of a new set of genes, the majority of which are regulated by the changes in ecdysteroid levels. We have initiated studies to identify genes whose expression is up-regulated between 24 and 4 h before pupal ecdysis in Manduca sexta. In this paper we report the partial characterization of one such gene, esr16. The transcript of esr16 is detected by Northern blot analysis in nervous tissue, muscle and trachea isolated from animals 4 h before, but not 24 h before pupal ecdysis. In situ hybridization showed that the transcript was expressed in epithelial cells of the large tracheae surrounding the nervous system and muscle. Sequence analysis suggested that the gene encoded a secreted protein with 35{\%} identity to HE1, a human epididymal-specific gene.",
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