Identification of a Cryptococcus neoformans gene that directs expression of the cryptic Saccharomyces cerevisiae mannitol dehydrogenase gene

John R. Perfect, Thomas H. Rude, Brian Wong, Timothy Flynn, Vishnu Chaturvedi, Walter Niehaus

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The Mtl gene from Cryptococcus neoformans, which confers the ability of Saccharomyces cerevisiae Sc41 YJO to grow on mannitol with substantial NAD- dependent mannitol dehydrogenase activity, was identified. Purifications and characterizations of this enzyme show that it is found in polyploid strain BB1, and the peptide sequence of the enzyme helped identify the saccharomyces gene encoding this mannitol dehydrogenase activity. On the other hand, the Mtl gene of C. neoformans encodes a 346-amino-acid protein which is not mannitol dehydrogenase but a regulatory element which is active in a heterologous fungus.

Original languageEnglish (US)
Pages (from-to)5257-5262
Number of pages6
JournalJournal of bacteriology
Volume178
Issue number17
DOIs
StatePublished - Jan 1 1996

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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