Identification of a Cryptococcus neoformans gene that directs expression of the cryptic Saccharomyces cerevisiae mannitol dehydrogenase gene

John R. Perfect, Thomas H. Rude, Brian Wong, Timothy Flynn, Vishnu Chaturvedi, Walter Niehaus

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The Mtl gene from Cryptococcus neoformans, which confers the ability of Saccharomyces cerevisiae Sc41 YJO to grow on mannitol with substantial NAD- dependent mannitol dehydrogenase activity, was identified. Purifications and characterizations of this enzyme show that it is found in polyploid strain BB1, and the peptide sequence of the enzyme helped identify the saccharomyces gene encoding this mannitol dehydrogenase activity. On the other hand, the Mtl gene of C. neoformans encodes a 346-amino-acid protein which is not mannitol dehydrogenase but a regulatory element which is active in a heterologous fungus.

Original languageEnglish (US)
Pages (from-to)5257-5262
Number of pages6
JournalJournal of Bacteriology
Volume178
Issue number17
StatePublished - 1996
Externally publishedYes

Fingerprint

Mannitol Dehydrogenases
Cryptococcus neoformans
Saccharomyces cerevisiae
Gene Expression
Genes
Saccharomyces
Polyploidy
Mannitol
Enzymes
NAD
Fungi
Amino Acids
Peptides
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Perfect, J. R., Rude, T. H., Wong, B., Flynn, T., Chaturvedi, V., & Niehaus, W. (1996). Identification of a Cryptococcus neoformans gene that directs expression of the cryptic Saccharomyces cerevisiae mannitol dehydrogenase gene. Journal of Bacteriology, 178(17), 5257-5262.

Identification of a Cryptococcus neoformans gene that directs expression of the cryptic Saccharomyces cerevisiae mannitol dehydrogenase gene. / Perfect, John R.; Rude, Thomas H.; Wong, Brian; Flynn, Timothy; Chaturvedi, Vishnu; Niehaus, Walter.

In: Journal of Bacteriology, Vol. 178, No. 17, 1996, p. 5257-5262.

Research output: Contribution to journalArticle

Perfect, JR, Rude, TH, Wong, B, Flynn, T, Chaturvedi, V & Niehaus, W 1996, 'Identification of a Cryptococcus neoformans gene that directs expression of the cryptic Saccharomyces cerevisiae mannitol dehydrogenase gene', Journal of Bacteriology, vol. 178, no. 17, pp. 5257-5262.
Perfect, John R. ; Rude, Thomas H. ; Wong, Brian ; Flynn, Timothy ; Chaturvedi, Vishnu ; Niehaus, Walter. / Identification of a Cryptococcus neoformans gene that directs expression of the cryptic Saccharomyces cerevisiae mannitol dehydrogenase gene. In: Journal of Bacteriology. 1996 ; Vol. 178, No. 17. pp. 5257-5262.
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