Abstract
We have isolated and characterized a unique gene that encodes a highly conserved membrane bound extracellular protein that defines a new epidermal growth factor-related gene family. The CRELD1 (Cysteine-Rich with EGF-Like Domains 1) gene (previously known as cirrin) was cloned from a human chromosome 3 BAC. Mapping of the gene confirmed its position at chromosome 3p25.3. The gene is ubiquitously expressed in early development and later becomes more markedly expressed in the developing heart, limb buds, mandible and central nervous system. Expression persists in adulthood in most tissues. Sequence analysis suggests that this is a cell adhesion protein. The mouse orthologue was cloned and mapped to the syntenic region of mouse chromosome 6. Orthologues or homologues have also been identified for cow, Chinese hamster, Drosophila and Caenorhabditis elegans. The CRELD1 gene is deleted in the human cytogenetic disorder 3p- syndrome and is in the region of loss of heterozygosity for several types of cancer. A potential role for this protein in these disorders is discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 47-57 |
| Number of pages | 11 |
| Journal | Gene |
| Volume | 293 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Jun 26 2002 |
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Keywords
- Cell adhesion
- Chromosome 3p25
- Cirrin, EGF superfamily
- Extracellular
- Transmembrane domain
ASJC Scopus subject areas
- Genetics
Cite this
Identification, genomic organization and mRNA expression of CRELD1, the founding member of a unique family of matricellular proteins. / Rupp, Paul A.; Fouad, Gameil T.; Egelston, Carley A.; Reifsteck, Carol A.; Olson, Susan; Knosp, Wendy M.; Glanville, Robert W.; Thornburg, Kent; Robinson, Susan W.; Maslen, Cheryl.
In: Gene, Vol. 293, No. 1-2, 26.06.2002, p. 47-57.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification, genomic organization and mRNA expression of CRELD1, the founding member of a unique family of matricellular proteins
AU - Rupp, Paul A.
AU - Fouad, Gameil T.
AU - Egelston, Carley A.
AU - Reifsteck, Carol A.
AU - Olson, Susan
AU - Knosp, Wendy M.
AU - Glanville, Robert W.
AU - Thornburg, Kent
AU - Robinson, Susan W.
AU - Maslen, Cheryl
PY - 2002/6/26
Y1 - 2002/6/26
N2 - We have isolated and characterized a unique gene that encodes a highly conserved membrane bound extracellular protein that defines a new epidermal growth factor-related gene family. The CRELD1 (Cysteine-Rich with EGF-Like Domains 1) gene (previously known as cirrin) was cloned from a human chromosome 3 BAC. Mapping of the gene confirmed its position at chromosome 3p25.3. The gene is ubiquitously expressed in early development and later becomes more markedly expressed in the developing heart, limb buds, mandible and central nervous system. Expression persists in adulthood in most tissues. Sequence analysis suggests that this is a cell adhesion protein. The mouse orthologue was cloned and mapped to the syntenic region of mouse chromosome 6. Orthologues or homologues have also been identified for cow, Chinese hamster, Drosophila and Caenorhabditis elegans. The CRELD1 gene is deleted in the human cytogenetic disorder 3p- syndrome and is in the region of loss of heterozygosity for several types of cancer. A potential role for this protein in these disorders is discussed.
AB - We have isolated and characterized a unique gene that encodes a highly conserved membrane bound extracellular protein that defines a new epidermal growth factor-related gene family. The CRELD1 (Cysteine-Rich with EGF-Like Domains 1) gene (previously known as cirrin) was cloned from a human chromosome 3 BAC. Mapping of the gene confirmed its position at chromosome 3p25.3. The gene is ubiquitously expressed in early development and later becomes more markedly expressed in the developing heart, limb buds, mandible and central nervous system. Expression persists in adulthood in most tissues. Sequence analysis suggests that this is a cell adhesion protein. The mouse orthologue was cloned and mapped to the syntenic region of mouse chromosome 6. Orthologues or homologues have also been identified for cow, Chinese hamster, Drosophila and Caenorhabditis elegans. The CRELD1 gene is deleted in the human cytogenetic disorder 3p- syndrome and is in the region of loss of heterozygosity for several types of cancer. A potential role for this protein in these disorders is discussed.
KW - Cell adhesion
KW - Chromosome 3p25
KW - Cirrin, EGF superfamily
KW - Extracellular
KW - Transmembrane domain
UR - http://www.scopus.com/inward/record.url?scp=0037178268&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037178268&partnerID=8YFLogxK
U2 - 10.1016/S0378-1119(02)00696-0
DO - 10.1016/S0378-1119(02)00696-0
M3 - Article
C2 - 12137942
AN - SCOPUS:0037178268
VL - 293
SP - 47
EP - 57
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1-2
ER -