Identification and secretion of gamma-endorphin and beta-endorphin-like peptides from the neurointermediate pituitary of the rat.

Daniel Dorsa, L. D. Keith

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2 Citations (Scopus)

Abstract

Gamma-endorphin (gamma E) and related peptides were chromatographically identified in extracts of neurointermediate (NIL) pituitary. When extracts of this tissue were purified and subjected to HPLC in order to identify gamma E-related peptides gamma E, des-tyrosine gamma E, des-enkephalin-gamma E, and alpha-N acetyl gamma E co-elutable peptides were evident. Perifusates of dispersed NIL tissue were also shown to contain beta E and gamma E-immunoreactivity, and the secretion of both was suppressed by exposure of the tissue to dopamine. This study provides evidence of the endogenous presence and secretion of pharmacologically active gamma E-related peptides by the intermediate lobe of the rat pituitary.

Original languageEnglish (US)
Pages (from-to)285-292
Number of pages8
JournalProgress in Clinical and Biological Research
Volume192
StatePublished - 1985
Externally publishedYes

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gamma-Endorphin
beta-Endorphin
Peptides
Intermediate Pituitary Gland
Tissue Extracts
Tyrosine
Dopamine
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "Identification and secretion of gamma-endorphin and beta-endorphin-like peptides from the neurointermediate pituitary of the rat.",
abstract = "Gamma-endorphin (gamma E) and related peptides were chromatographically identified in extracts of neurointermediate (NIL) pituitary. When extracts of this tissue were purified and subjected to HPLC in order to identify gamma E-related peptides gamma E, des-tyrosine gamma E, des-enkephalin-gamma E, and alpha-N acetyl gamma E co-elutable peptides were evident. Perifusates of dispersed NIL tissue were also shown to contain beta E and gamma E-immunoreactivity, and the secretion of both was suppressed by exposure of the tissue to dopamine. This study provides evidence of the endogenous presence and secretion of pharmacologically active gamma E-related peptides by the intermediate lobe of the rat pituitary.",
author = "Daniel Dorsa and Keith, {L. D.}",
year = "1985",
language = "English (US)",
volume = "192",
pages = "285--292",
journal = "Progress in Clinical and Biological Research",
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T1 - Identification and secretion of gamma-endorphin and beta-endorphin-like peptides from the neurointermediate pituitary of the rat.

AU - Dorsa, Daniel

AU - Keith, L. D.

PY - 1985

Y1 - 1985

N2 - Gamma-endorphin (gamma E) and related peptides were chromatographically identified in extracts of neurointermediate (NIL) pituitary. When extracts of this tissue were purified and subjected to HPLC in order to identify gamma E-related peptides gamma E, des-tyrosine gamma E, des-enkephalin-gamma E, and alpha-N acetyl gamma E co-elutable peptides were evident. Perifusates of dispersed NIL tissue were also shown to contain beta E and gamma E-immunoreactivity, and the secretion of both was suppressed by exposure of the tissue to dopamine. This study provides evidence of the endogenous presence and secretion of pharmacologically active gamma E-related peptides by the intermediate lobe of the rat pituitary.

AB - Gamma-endorphin (gamma E) and related peptides were chromatographically identified in extracts of neurointermediate (NIL) pituitary. When extracts of this tissue were purified and subjected to HPLC in order to identify gamma E-related peptides gamma E, des-tyrosine gamma E, des-enkephalin-gamma E, and alpha-N acetyl gamma E co-elutable peptides were evident. Perifusates of dispersed NIL tissue were also shown to contain beta E and gamma E-immunoreactivity, and the secretion of both was suppressed by exposure of the tissue to dopamine. This study provides evidence of the endogenous presence and secretion of pharmacologically active gamma E-related peptides by the intermediate lobe of the rat pituitary.

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