TY - JOUR
T1 - Identification and quantitation of drugs of abuse in urine using the generalized rank annihilation method of curve resolution
AU - Li, Shousong
AU - Gemperline, Paul J.
AU - Briley, Kimberly
AU - Kazmierczak, Steven
PY - 1994/5/13
Y1 - 1994/5/13
N2 - A rapid analytical method which is of practical use for the identification and quantitation of drugs of abuse in urine using HPLC with a diode-array detection is described. Because the method utilizes mathematical resolution of partially resolved peaks, greatly simplified sample preparation procedures and very short run times can be used. The generalized rank annihilation method (GRAM) is used to eliminate response due to unknown background peaks and separate partially resolved peaks. An optimized gradient elution program was found for which morphine, phenylpropanolamine, ephedrine, benzoylecgonine, lidocaine, cocaine, diphenhydramine, nortriptyline, norpropoxyphene, nordiazepam, codeine, d-amphetamine, meperidine, and amitriptyline elute from the HPLC column in less than 8.5 min. A commercially available system for HPLC analysis of drugs of abuse is currently available, however, the commercially available system takes 21 min to complete its analysis. Two modified sample pre-treatment methods were also developed to simplify sample treatment procedures substantially. In this paper, The GRAM technique is shown to be extremely powerful in identifying drugs of abuse from large overlapping peaks.
AB - A rapid analytical method which is of practical use for the identification and quantitation of drugs of abuse in urine using HPLC with a diode-array detection is described. Because the method utilizes mathematical resolution of partially resolved peaks, greatly simplified sample preparation procedures and very short run times can be used. The generalized rank annihilation method (GRAM) is used to eliminate response due to unknown background peaks and separate partially resolved peaks. An optimized gradient elution program was found for which morphine, phenylpropanolamine, ephedrine, benzoylecgonine, lidocaine, cocaine, diphenhydramine, nortriptyline, norpropoxyphene, nordiazepam, codeine, d-amphetamine, meperidine, and amitriptyline elute from the HPLC column in less than 8.5 min. A commercially available system for HPLC analysis of drugs of abuse is currently available, however, the commercially available system takes 21 min to complete its analysis. Two modified sample pre-treatment methods were also developed to simplify sample treatment procedures substantially. In this paper, The GRAM technique is shown to be extremely powerful in identifying drugs of abuse from large overlapping peaks.
UR - http://www.scopus.com/inward/record.url?scp=0028286159&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028286159&partnerID=8YFLogxK
U2 - 10.1016/0378-4347(94)00102-2
DO - 10.1016/0378-4347(94)00102-2
M3 - Article
C2 - 8081467
AN - SCOPUS:0028286159
SN - 0378-4347
VL - 655
SP - 213
EP - 223
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 2
ER -