Identification and expression of a human cytomegalovirus early glycoprotein

C. P. Chang, D. H. Vesole, Jay Nelson, M. B A Oldstone, M. F. Stinski

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A human cytomegalovirus early gene which possesses three temporally regulated promoters is located in the large unique component of the viral genome between 0.054 and 0.064 map units (C.-P. Chang, C. L. Malone, and M. F. Stinski, J. Virol. 63: 281-290, 1989). This gene contains a major open reading frame (ORF) located 233 bases downstream of the cap site of an early unspliced RNA. The major ORF predicts a polypeptide of 17 kilodaltons (kDa) which contains a glycoproteinlike signal and anchor domains as well as potential N-glycosylation sites. Antisera were prepared against synthetic peptides derived from amino acid sequences within the major ORF. The antisera detected a viral glycoprotein of 48 kDa in infected cells and recognized the in vitro-translated 17-kDa protein early-gene product. The viral glycoprotein, designated gp48, was modified by N-linked glycans and possibly O-linked glycans. The synthesis of gp48 occurred in the absence of viral DNA replication but accumulated to the highest levels at late times after infection. Since gp48 was found in the virion, it is considered an early structural glycoprotein.

Original languageEnglish (US)
Pages (from-to)3330-3337
Number of pages8
JournalJournal of Virology
Volume63
Issue number8
StatePublished - 1989
Externally publishedYes

Fingerprint

Human herpesvirus 5
Cytomegalovirus
Open Reading Frames
open reading frames
glycoproteins
Glycoproteins
Polysaccharides
antiserum
Immune Sera
polysaccharides
Peptides
synthetic peptides
Viral Genome
Viral DNA
DNA replication
major genes
glycosylation
DNA Replication
Glycosylation
virion

ASJC Scopus subject areas

  • Immunology

Cite this

Chang, C. P., Vesole, D. H., Nelson, J., Oldstone, M. B. A., & Stinski, M. F. (1989). Identification and expression of a human cytomegalovirus early glycoprotein. Journal of Virology, 63(8), 3330-3337.

Identification and expression of a human cytomegalovirus early glycoprotein. / Chang, C. P.; Vesole, D. H.; Nelson, Jay; Oldstone, M. B A; Stinski, M. F.

In: Journal of Virology, Vol. 63, No. 8, 1989, p. 3330-3337.

Research output: Contribution to journalArticle

Chang, CP, Vesole, DH, Nelson, J, Oldstone, MBA & Stinski, MF 1989, 'Identification and expression of a human cytomegalovirus early glycoprotein', Journal of Virology, vol. 63, no. 8, pp. 3330-3337.
Chang CP, Vesole DH, Nelson J, Oldstone MBA, Stinski MF. Identification and expression of a human cytomegalovirus early glycoprotein. Journal of Virology. 1989;63(8):3330-3337.
Chang, C. P. ; Vesole, D. H. ; Nelson, Jay ; Oldstone, M. B A ; Stinski, M. F. / Identification and expression of a human cytomegalovirus early glycoprotein. In: Journal of Virology. 1989 ; Vol. 63, No. 8. pp. 3330-3337.
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