Identification and cloning of a novel monocarboxylate transporter gene

Yu Qiang Ge, Chao-Ling Yang, S. Aronson Peter

Research output: Contribution to journalArticle

Abstract

Purpose: To identify and clone the cDNAs encoding renal formate and (or) oxalate transporters in order to investigate the mechanism of Cl- reabsorption in the proximal tubule. Methods: According to the sequence of a bacterial formate - oxalate exchanger (Oxlt), we tested whether the mammalian EST database contained a kidney cDNA with similarity to Oxlt. The gene was analyzed to locate the distribution with a mouse multiple-tissues Northern blot. Results: An EST with significant similarity to Oxlt was identified in a mouse renal cDNA library. The sequence of this EST clone had an open reading frame encoding a predicted protein of 429 amino acids. Database searching with the coding sequence revealed significant homology to the MCT family of monocarboxylate transporter (MCT). There were 33% identity with mouse MCT2,30% with mouse MCT1 and 29% with rat MCT3 in amino acid sequence. Amino acid 14-158 were 25% identical to the corresponding sequence of Oxlt. Northern blot analysis of mouse multiple-tissue indicated that transcript encoding novel protein highly expressed in kidney. There were barely detectable expression in liver and heart. Conclusions: A novel MCT family member with selection expression in the kidney was identified. It may be a candidate to mediate renal tubular formate transport.

Original languageEnglish (US)
Pages (from-to)469-472
Number of pages4
JournalFudan University Journal of Medical Sciences
Volume28
Issue number6
StatePublished - 2001
Externally publishedYes

Fingerprint

formic acid
Organism Cloning
Kidney
Expressed Sequence Tags
Genes
Oxalates
Northern Blotting
Complementary DNA
Clone Cells
Databases
Amino Acids
Gene Library
Open Reading Frames
Amino Acid Sequence
Proteins
Liver

Keywords

  • Cloning
  • Monocarboxylate
  • Transporter

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Identification and cloning of a novel monocarboxylate transporter gene. / Ge, Yu Qiang; Yang, Chao-Ling; Peter, S. Aronson.

In: Fudan University Journal of Medical Sciences, Vol. 28, No. 6, 2001, p. 469-472.

Research output: Contribution to journalArticle

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