Identification and cloning of a novel monocarboxylate transporter gene

Yu Qiang Ge, Chao Ling Yang, S. Aronson Peter

Research output: Contribution to journalArticle

Abstract

Purpose: To identify and clone the cDNAs encoding renal formate and (or) oxalate transporters in order to investigate the mechanism of Cl- reabsorption in the proximal tubule. Methods: According to the sequence of a bacterial formate - oxalate exchanger (Oxlt), we tested whether the mammalian EST database contained a kidney cDNA with similarity to Oxlt. The gene was analyzed to locate the distribution with a mouse multiple-tissues Northern blot. Results: An EST with significant similarity to Oxlt was identified in a mouse renal cDNA library. The sequence of this EST clone had an open reading frame encoding a predicted protein of 429 amino acids. Database searching with the coding sequence revealed significant homology to the MCT family of monocarboxylate transporter (MCT). There were 33% identity with mouse MCT2,30% with mouse MCT1 and 29% with rat MCT3 in amino acid sequence. Amino acid 14-158 were 25% identical to the corresponding sequence of Oxlt. Northern blot analysis of mouse multiple-tissue indicated that transcript encoding novel protein highly expressed in kidney. There were barely detectable expression in liver and heart. Conclusions: A novel MCT family member with selection expression in the kidney was identified. It may be a candidate to mediate renal tubular formate transport.

Original languageEnglish (US)
Pages (from-to)469-472
Number of pages4
JournalFudan University Journal of Medical Sciences
Volume28
Issue number6
StatePublished - Jan 1 2001

Keywords

  • Cloning
  • Monocarboxylate
  • Transporter

ASJC Scopus subject areas

  • Medicine(all)

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