Identification and characterization of two novel SH2 domain-containing proteins from a yeast two hybrid screen with the ABL tyrosine kinase

Tsukasa Oda, Jody Kujovich, Margaret Reis, Brenda Newman, Brian Druker

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

To further our understanding of the molecular mechanism of Bcr-Abl mediated transformation, a yeast two hybrid screen was used to identify proteins binding to the Abl tyrosine kinase. Two partial cDNAs encoding novel SH2 domain-containing proteins were cloned and designated Shd and She. Both have homology to Shb, a previously reported SH2 domain-containing protein. Northern blot analysis showed that She is expressed in heart, lung, brain, and skeletal muscle, while expression of Shd is restricted to the brain. The deduced amino acid sequence of the full length mouse Shd cDNA contains an amino-terminal proline-rich region, and a carboxy-terminal SH2 domain. A bacterially expressed Shd domain bound multiple tyrosine-phosphorylated proteins with relative molecular weights of 200, 170, 130, 100, 90, 78, 72 and 32 kDa from K562 cell lysates. Shd contains five YXXP motifs, a substrate sequence preferred by Abl tyrosine kinases. Shd was tyrosine phosphorylated in COS-7 cells co-transfected with Shd and c-Abl or Bcr-Abl. These results suggest that Shd may be a physiological substrate of c-Abl and may function as an adapter protein in the central nervous system.

Original languageEnglish (US)
Pages (from-to)1255-1262
Number of pages8
JournalOncogene
Volume15
Issue number11
StatePublished - 1997

Fingerprint

Fungal Proteins
src Homology Domains
Protein-Tyrosine Kinases
Tyrosine
Proteins
Complementary DNA
K562 Cells
COS Cells
Brain
Proline
Protein Binding
Northern Blotting
Amino Acid Sequence
Skeletal Muscle
Central Nervous System
Yeasts
Molecular Weight
Lung

Keywords

  • c-Abl
  • SH2 domains
  • Tyrosine kinase
  • Yeast two hybird screen

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Identification and characterization of two novel SH2 domain-containing proteins from a yeast two hybrid screen with the ABL tyrosine kinase. / Oda, Tsukasa; Kujovich, Jody; Reis, Margaret; Newman, Brenda; Druker, Brian.

In: Oncogene, Vol. 15, No. 11, 1997, p. 1255-1262.

Research output: Contribution to journalArticle

Oda, Tsukasa ; Kujovich, Jody ; Reis, Margaret ; Newman, Brenda ; Druker, Brian. / Identification and characterization of two novel SH2 domain-containing proteins from a yeast two hybrid screen with the ABL tyrosine kinase. In: Oncogene. 1997 ; Vol. 15, No. 11. pp. 1255-1262.
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