Identification and characterization of primordial germ cells in a vocal learning Neoaves species, the zebra finch

Kyung Min Jung, Young Min Kim, Anna L. Keyte, Matthew T. Biegler, Deivendran Rengaraj, Hong Jo Lee, Claudio V. Mello, Tarciso A.F. Velho, Olivier Fedrigo, Bettina Haase, Erich D. Jarvis, Jae Yong Han

Research output: Contribution to journalArticle

Abstract

The zebra finch has been used as a valuable vocal learning animal model for human spoken language. It is representative of vocal learning songbirds specifically, which comprise half of all bird species, and of Neoaves broadly, which comprise 95% of all bird species. Although transgenesis in the zebra finch has been accomplished, it is with a very low efficiency of germ-line transmission and far from the efficiency with a more genetically tractable but vocal nonlearning species, the chicken (a Galloanseriformes). To improve germ-line transmission in the zebra finch, we identified and characterized its primordial germ cells (PGCs) and compared them with chicken. We found striking differences between the 2 species, including that zebra finch PGCs were more numerous, more widely distributed in early embryos before colonization into the gonads, had slower timing of colonization, and had a different developmental gene-expression program. We improved conditions for isolating and culturing zebra finch PGCs in vitro and were able to transfect them with gene-expression vectors and incorporate them into the gonads of host embryos. Our findings demonstrate important differences in the PGCs of the zebra finch and advance the first stage of creating PGC-mediated germ-line transgenics of a vocal learning species.-Jung, K. M., Kim, Y. M., Keyte, A. L., Biegler, M. T., Rengaraj, D., Lee, H. J., Mello, C. V., Velho, T. A. F., Fedrigo, O., Haase, B., Jarvis, E. D., Han, J. Y. Identification and characterization of primordial germ cells in a vocal learning Neoaves species, the zebra finch.

Original languageEnglish (US)
Pages (from-to)13825-13836
Number of pages12
JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume33
Issue number12
DOIs
StatePublished - Dec 1 2019

Fingerprint

Finches
Equidae
Birds
Gene expression
Germ Cells
Electric lines
Cells
Learning
Animals
Gonads
Chickens
Embryonic Structures
Developmental Genes
Efficiency
Gene Expression
Gene Transfer Techniques
Songbirds
Language
Animal Models

Keywords

  • DAZL
  • embryo development
  • germ cells
  • songbird
  • transgenesis

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Identification and characterization of primordial germ cells in a vocal learning Neoaves species, the zebra finch. / Jung, Kyung Min; Kim, Young Min; Keyte, Anna L.; Biegler, Matthew T.; Rengaraj, Deivendran; Lee, Hong Jo; Mello, Claudio V.; Velho, Tarciso A.F.; Fedrigo, Olivier; Haase, Bettina; Jarvis, Erich D.; Han, Jae Yong.

In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, No. 12, 01.12.2019, p. 13825-13836.

Research output: Contribution to journalArticle

Jung, Kyung Min ; Kim, Young Min ; Keyte, Anna L. ; Biegler, Matthew T. ; Rengaraj, Deivendran ; Lee, Hong Jo ; Mello, Claudio V. ; Velho, Tarciso A.F. ; Fedrigo, Olivier ; Haase, Bettina ; Jarvis, Erich D. ; Han, Jae Yong. / Identification and characterization of primordial germ cells in a vocal learning Neoaves species, the zebra finch. In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2019 ; Vol. 33, No. 12. pp. 13825-13836.
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