Identification and characterization of a novel β subunit of soluble guanylyl cyclase that is active in the absence of a second subunit and is relatively insensitive to nitric oxide

Alan Nighorn, Kathryn A. Byrnes, David Morton

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44 Citations (Scopus)

Abstract

Previously characterized soluble guanylyl cyclases form α-β heterodimers that can be activated by the gaseous messenger, nitric oxide. In mammals, four subunits have been cloned, named α1, α2, β1, and β2. We have identified a novel soluble guanylyl cyclase isoform from the nervous system of the insect Manduca sexta that we have named M. sexta guanylyl cyclase β3 (MsGC-β3). It is most closely related to the mammalian β subunits but has several features that distinguish it from previously identified soluble cyclases. Most importantly, MsGC-β3 does not need to form heterodimers to form an active enzyme because guanylyl cyclase activity can be measured when it is expressed alone in COS-7 cells. Moreover, this activity is only weakly enhanced in the presence of the nitric oxide donor, sodium nitroprusside. Several of the amino acids in rat β1 subunits, previously identified as being important in heme binding or necessary for nitric oxide activation, are substituted with nonsimilar amino acids in MsGC- β3. There are also an additional 315 amino acids C-terminal to the catalytic domain of MsGC-β3 that have no sequence similarity to any known protein. Northern blot analysis shows that MsGC-β3 is primarily expressed in the nervous system of Manduca.

Original languageEnglish (US)
Pages (from-to)2525-2531
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number4
DOIs
StatePublished - Jan 22 1999

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Manduca
Guanylate Cyclase
Nitric Oxide
Amino Acids
Neurology
Nervous System
Nitric Oxide Donors
COS Cells
Nitroprusside
Mammals
Heme
Northern Blotting
Insects
Soluble Guanylyl Cyclase
Catalytic Domain
Protein Isoforms
Rats
Chemical activation
Enzymes
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

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title = "Identification and characterization of a novel β subunit of soluble guanylyl cyclase that is active in the absence of a second subunit and is relatively insensitive to nitric oxide",
abstract = "Previously characterized soluble guanylyl cyclases form α-β heterodimers that can be activated by the gaseous messenger, nitric oxide. In mammals, four subunits have been cloned, named α1, α2, β1, and β2. We have identified a novel soluble guanylyl cyclase isoform from the nervous system of the insect Manduca sexta that we have named M. sexta guanylyl cyclase β3 (MsGC-β3). It is most closely related to the mammalian β subunits but has several features that distinguish it from previously identified soluble cyclases. Most importantly, MsGC-β3 does not need to form heterodimers to form an active enzyme because guanylyl cyclase activity can be measured when it is expressed alone in COS-7 cells. Moreover, this activity is only weakly enhanced in the presence of the nitric oxide donor, sodium nitroprusside. Several of the amino acids in rat β1 subunits, previously identified as being important in heme binding or necessary for nitric oxide activation, are substituted with nonsimilar amino acids in MsGC- β3. There are also an additional 315 amino acids C-terminal to the catalytic domain of MsGC-β3 that have no sequence similarity to any known protein. Northern blot analysis shows that MsGC-β3 is primarily expressed in the nervous system of Manduca.",
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T1 - Identification and characterization of a novel β subunit of soluble guanylyl cyclase that is active in the absence of a second subunit and is relatively insensitive to nitric oxide

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AU - Morton, David

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N2 - Previously characterized soluble guanylyl cyclases form α-β heterodimers that can be activated by the gaseous messenger, nitric oxide. In mammals, four subunits have been cloned, named α1, α2, β1, and β2. We have identified a novel soluble guanylyl cyclase isoform from the nervous system of the insect Manduca sexta that we have named M. sexta guanylyl cyclase β3 (MsGC-β3). It is most closely related to the mammalian β subunits but has several features that distinguish it from previously identified soluble cyclases. Most importantly, MsGC-β3 does not need to form heterodimers to form an active enzyme because guanylyl cyclase activity can be measured when it is expressed alone in COS-7 cells. Moreover, this activity is only weakly enhanced in the presence of the nitric oxide donor, sodium nitroprusside. Several of the amino acids in rat β1 subunits, previously identified as being important in heme binding or necessary for nitric oxide activation, are substituted with nonsimilar amino acids in MsGC- β3. There are also an additional 315 amino acids C-terminal to the catalytic domain of MsGC-β3 that have no sequence similarity to any known protein. Northern blot analysis shows that MsGC-β3 is primarily expressed in the nervous system of Manduca.

AB - Previously characterized soluble guanylyl cyclases form α-β heterodimers that can be activated by the gaseous messenger, nitric oxide. In mammals, four subunits have been cloned, named α1, α2, β1, and β2. We have identified a novel soluble guanylyl cyclase isoform from the nervous system of the insect Manduca sexta that we have named M. sexta guanylyl cyclase β3 (MsGC-β3). It is most closely related to the mammalian β subunits but has several features that distinguish it from previously identified soluble cyclases. Most importantly, MsGC-β3 does not need to form heterodimers to form an active enzyme because guanylyl cyclase activity can be measured when it is expressed alone in COS-7 cells. Moreover, this activity is only weakly enhanced in the presence of the nitric oxide donor, sodium nitroprusside. Several of the amino acids in rat β1 subunits, previously identified as being important in heme binding or necessary for nitric oxide activation, are substituted with nonsimilar amino acids in MsGC- β3. There are also an additional 315 amino acids C-terminal to the catalytic domain of MsGC-β3 that have no sequence similarity to any known protein. Northern blot analysis shows that MsGC-β3 is primarily expressed in the nervous system of Manduca.

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