Identification a human centrosomal calmodulin-binding protein that shares homology with pericentrin

Mark Flory, M. J. Moser, Jr Monnat R.J., T. N. Davis

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Eukaryotic chromosome segregation depends on the mitotic spindle apparatus, a bipolar array of microtubules nucleated from centrosomes. Centrosomal microtubule nucleation requires attachment of γ-tubulin ring complexes to a salt-insoluble centrosomal core, but the factor(s) underlying this attachment remains unknown. In budding yeast, this attachment is provided by the coiled-coil protein Spc110p, which links the yeast γ-tubulin complex to the core of the yeast centrosome. Here, we show that the large coiled-coil protein kendrin is a human orthologue of Spc110p. We identified kendrin by its C-terminal calmodulin-binding site, which shares homology with the Spc110p calmodulin-binding site. Kendrin localizes specifically to centrosomes throughout the cell cycle. N-terminal regions of kendrin share significant sequence homology with pericentrin, a previously identified murine centrosome component known to interact with γ-tubulin. In mitotic human breast carcinoma cells containing abundant centrosome-like structures, kendrin is found only at centrosomes associated with spindle microtubules.

Original languageEnglish (US)
Pages (from-to)5919-5923
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number11
DOIs
StatePublished - May 23 2000
Externally publishedYes

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Calmodulin-Binding Proteins
Forensic Anthropology
Centrosome
Tubulin
Microtubules
Calmodulin
Yeasts
Binding Sites
Chromosome Segregation
Spindle Apparatus
Saccharomycetales
Sequence Homology
pericentrin
Cell Cycle
Proteins
Salts
kendrin
Breast Neoplasms

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Identification a human centrosomal calmodulin-binding protein that shares homology with pericentrin. / Flory, Mark; Moser, M. J.; Monnat R.J., Jr; Davis, T. N.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 11, 23.05.2000, p. 5919-5923.

Research output: Contribution to journalArticle

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