ICP-MS/MS-Based Ionomics

A Validated Methodology to Investigate the Biological Variability of the Human Ionome

Tobias Konz, Eugenia Migliavacca, Loïc Dayon, Gene Bowman, Aikaterini Oikonomidi, Julius Popp, Serge Rezzi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We here describe the development, validation and application of a quantitative methodology for the simultaneous determination of 29 elements in human serum using state-of-the-art inductively coupled plasma triple quadrupole mass spectrometry (ICP-MS/MS). This new methodology offers high-throughput elemental profiling using simple dilution of minimal quantity of serum samples. We report the outcomes of the validation procedure including limits of detection/quantification, linearity of calibration curves, precision, recovery and measurement uncertainty. ICP-MS/MS-based ionomics was used to analyze human serum of 120 older adults. Following a metabolomic data mining approach, the generated ionome profiles were subjected to principal component analysis revealing gender and age-specific differences. The ionome of female individuals was marked by higher levels of calcium, phosphorus, copper and copper to zinc ratio, while iron concentration was lower with respect to male subjects. Age was associated with lower concentrations of zinc. These findings were complemented with additional readouts to interpret micronutrient status including ceruloplasmin, ferritin and inorganic phosphate. Our data supports a gender-specific compartmentalization of the ionome that may reflect different bone remodelling in female individuals. Our ICP-MS/MS methodology enriches the panel of validated "Omics" approaches to study molecular relationships between the exposome and the ionome in relation with nutrition and health.

Original languageEnglish (US)
Pages (from-to)2080-2090
Number of pages11
JournalJournal of Proteome Research
Volume16
Issue number5
DOIs
StatePublished - May 5 2017
Externally publishedYes

Fingerprint

Inductively coupled plasma
Zinc
Copper
Ceruloplasmin
Micronutrients
Ferritins
Nutrition
Serum
Principal component analysis
Phosphorus
Dilution
Mass spectrometry
Data mining
Bone
Metabolomics
Data Mining
Iron
Bone Remodeling
Phosphates
Throughput

Keywords

  • ionome
  • ionomics
  • multielemental profiling
  • triple quadrupole ICP-MS

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

ICP-MS/MS-Based Ionomics : A Validated Methodology to Investigate the Biological Variability of the Human Ionome. / Konz, Tobias; Migliavacca, Eugenia; Dayon, Loïc; Bowman, Gene; Oikonomidi, Aikaterini; Popp, Julius; Rezzi, Serge.

In: Journal of Proteome Research, Vol. 16, No. 5, 05.05.2017, p. 2080-2090.

Research output: Contribution to journalArticle

Konz, T, Migliavacca, E, Dayon, L, Bowman, G, Oikonomidi, A, Popp, J & Rezzi, S 2017, 'ICP-MS/MS-Based Ionomics: A Validated Methodology to Investigate the Biological Variability of the Human Ionome', Journal of Proteome Research, vol. 16, no. 5, pp. 2080-2090. https://doi.org/10.1021/acs.jproteome.7b00055
Konz, Tobias ; Migliavacca, Eugenia ; Dayon, Loïc ; Bowman, Gene ; Oikonomidi, Aikaterini ; Popp, Julius ; Rezzi, Serge. / ICP-MS/MS-Based Ionomics : A Validated Methodology to Investigate the Biological Variability of the Human Ionome. In: Journal of Proteome Research. 2017 ; Vol. 16, No. 5. pp. 2080-2090.
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