TY - JOUR
T1 - ICP-MS/MS-Based Ionomics
T2 - A Validated Methodology to Investigate the Biological Variability of the Human Ionome
AU - Konz, Tobias
AU - Migliavacca, Eugenia
AU - Dayon, Loïc
AU - Bowman, Gene
AU - Oikonomidi, Aikaterini
AU - Popp, Julius
AU - Rezzi, Serge
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/5/5
Y1 - 2017/5/5
N2 - We here describe the development, validation and application of a quantitative methodology for the simultaneous determination of 29 elements in human serum using state-of-the-art inductively coupled plasma triple quadrupole mass spectrometry (ICP-MS/MS). This new methodology offers high-throughput elemental profiling using simple dilution of minimal quantity of serum samples. We report the outcomes of the validation procedure including limits of detection/quantification, linearity of calibration curves, precision, recovery and measurement uncertainty. ICP-MS/MS-based ionomics was used to analyze human serum of 120 older adults. Following a metabolomic data mining approach, the generated ionome profiles were subjected to principal component analysis revealing gender and age-specific differences. The ionome of female individuals was marked by higher levels of calcium, phosphorus, copper and copper to zinc ratio, while iron concentration was lower with respect to male subjects. Age was associated with lower concentrations of zinc. These findings were complemented with additional readouts to interpret micronutrient status including ceruloplasmin, ferritin and inorganic phosphate. Our data supports a gender-specific compartmentalization of the ionome that may reflect different bone remodelling in female individuals. Our ICP-MS/MS methodology enriches the panel of validated "Omics" approaches to study molecular relationships between the exposome and the ionome in relation with nutrition and health.
AB - We here describe the development, validation and application of a quantitative methodology for the simultaneous determination of 29 elements in human serum using state-of-the-art inductively coupled plasma triple quadrupole mass spectrometry (ICP-MS/MS). This new methodology offers high-throughput elemental profiling using simple dilution of minimal quantity of serum samples. We report the outcomes of the validation procedure including limits of detection/quantification, linearity of calibration curves, precision, recovery and measurement uncertainty. ICP-MS/MS-based ionomics was used to analyze human serum of 120 older adults. Following a metabolomic data mining approach, the generated ionome profiles were subjected to principal component analysis revealing gender and age-specific differences. The ionome of female individuals was marked by higher levels of calcium, phosphorus, copper and copper to zinc ratio, while iron concentration was lower with respect to male subjects. Age was associated with lower concentrations of zinc. These findings were complemented with additional readouts to interpret micronutrient status including ceruloplasmin, ferritin and inorganic phosphate. Our data supports a gender-specific compartmentalization of the ionome that may reflect different bone remodelling in female individuals. Our ICP-MS/MS methodology enriches the panel of validated "Omics" approaches to study molecular relationships between the exposome and the ionome in relation with nutrition and health.
KW - ionome
KW - ionomics
KW - multielemental profiling
KW - triple quadrupole ICP-MS
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U2 - 10.1021/acs.jproteome.7b00055
DO - 10.1021/acs.jproteome.7b00055
M3 - Article
C2 - 28383921
AN - SCOPUS:85019011786
SN - 1535-3893
VL - 16
SP - 2080
EP - 2090
JO - Journal of Proteome Research
JF - Journal of Proteome Research
IS - 5
ER -