Hypoxia induced preferential ketone utilization by rat brain slices

Jeffrey Kirsch, Louis G. D'Alecy

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

When exposed to hypoxia, intact mice, with elevated blood ketones, live longer than mice with normal blood ketones. To evaluate a possible mechanism responsible for this phenomenon a rat brain slice preparation was used to determine if brain tissue would utilize glucose or ketones preferentially during exposure to reduced oxygen. Reducing available oxygen in the incubation medium from 95%, in steps, to 5% produced the expected gradual reduction in the carbon dioxide formation from glucose. In contrast, reducing the oxygen level to 40 and 20% resulted in a statistically significant stimulation of the production of carbon dioxide from the ketone beta-hydroxybutyrate. At very low oxygen levels carbon dioxide production from either substrate was reduced. These results are consistent with the hypothesis that ketones can be used in addition to glucose as a substrate for brain energy production even during reduced oxygen availability. If the increase in carbon dioxide production from ketones can be equated with an increase in energy production from this supplemental substrate than ketones may be therapeutical!)' useful in avoiding the collapse of brain function during moderate hypoxia.

Original languageEnglish (US)
Pages (from-to)319-323
Number of pages5
JournalStroke
Volume15
Issue number2
StatePublished - 1984
Externally publishedYes

Fingerprint

Ketones
Carbon Dioxide
Brain
Oxygen
Glucose
3-Hydroxybutyric Acid
Hypoxia

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Advanced and Specialized Nursing
  • Neuroscience(all)

Cite this

Hypoxia induced preferential ketone utilization by rat brain slices. / Kirsch, Jeffrey; D'Alecy, Louis G.

In: Stroke, Vol. 15, No. 2, 1984, p. 319-323.

Research output: Contribution to journalArticle

Kirsch, J & D'Alecy, LG 1984, 'Hypoxia induced preferential ketone utilization by rat brain slices', Stroke, vol. 15, no. 2, pp. 319-323.
Kirsch, Jeffrey ; D'Alecy, Louis G. / Hypoxia induced preferential ketone utilization by rat brain slices. In: Stroke. 1984 ; Vol. 15, No. 2. pp. 319-323.
@article{704f345c0cb445b2a38e9d196e2e5e8f,
title = "Hypoxia induced preferential ketone utilization by rat brain slices",
abstract = "When exposed to hypoxia, intact mice, with elevated blood ketones, live longer than mice with normal blood ketones. To evaluate a possible mechanism responsible for this phenomenon a rat brain slice preparation was used to determine if brain tissue would utilize glucose or ketones preferentially during exposure to reduced oxygen. Reducing available oxygen in the incubation medium from 95{\%}, in steps, to 5{\%} produced the expected gradual reduction in the carbon dioxide formation from glucose. In contrast, reducing the oxygen level to 40 and 20{\%} resulted in a statistically significant stimulation of the production of carbon dioxide from the ketone beta-hydroxybutyrate. At very low oxygen levels carbon dioxide production from either substrate was reduced. These results are consistent with the hypothesis that ketones can be used in addition to glucose as a substrate for brain energy production even during reduced oxygen availability. If the increase in carbon dioxide production from ketones can be equated with an increase in energy production from this supplemental substrate than ketones may be therapeutical!)' useful in avoiding the collapse of brain function during moderate hypoxia.",
author = "Jeffrey Kirsch and D'Alecy, {Louis G.}",
year = "1984",
language = "English (US)",
volume = "15",
pages = "319--323",
journal = "Stroke",
issn = "0039-2499",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Hypoxia induced preferential ketone utilization by rat brain slices

AU - Kirsch, Jeffrey

AU - D'Alecy, Louis G.

PY - 1984

Y1 - 1984

N2 - When exposed to hypoxia, intact mice, with elevated blood ketones, live longer than mice with normal blood ketones. To evaluate a possible mechanism responsible for this phenomenon a rat brain slice preparation was used to determine if brain tissue would utilize glucose or ketones preferentially during exposure to reduced oxygen. Reducing available oxygen in the incubation medium from 95%, in steps, to 5% produced the expected gradual reduction in the carbon dioxide formation from glucose. In contrast, reducing the oxygen level to 40 and 20% resulted in a statistically significant stimulation of the production of carbon dioxide from the ketone beta-hydroxybutyrate. At very low oxygen levels carbon dioxide production from either substrate was reduced. These results are consistent with the hypothesis that ketones can be used in addition to glucose as a substrate for brain energy production even during reduced oxygen availability. If the increase in carbon dioxide production from ketones can be equated with an increase in energy production from this supplemental substrate than ketones may be therapeutical!)' useful in avoiding the collapse of brain function during moderate hypoxia.

AB - When exposed to hypoxia, intact mice, with elevated blood ketones, live longer than mice with normal blood ketones. To evaluate a possible mechanism responsible for this phenomenon a rat brain slice preparation was used to determine if brain tissue would utilize glucose or ketones preferentially during exposure to reduced oxygen. Reducing available oxygen in the incubation medium from 95%, in steps, to 5% produced the expected gradual reduction in the carbon dioxide formation from glucose. In contrast, reducing the oxygen level to 40 and 20% resulted in a statistically significant stimulation of the production of carbon dioxide from the ketone beta-hydroxybutyrate. At very low oxygen levels carbon dioxide production from either substrate was reduced. These results are consistent with the hypothesis that ketones can be used in addition to glucose as a substrate for brain energy production even during reduced oxygen availability. If the increase in carbon dioxide production from ketones can be equated with an increase in energy production from this supplemental substrate than ketones may be therapeutical!)' useful in avoiding the collapse of brain function during moderate hypoxia.

UR - http://www.scopus.com/inward/record.url?scp=0021348525&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021348525&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 319

EP - 323

JO - Stroke

JF - Stroke

SN - 0039-2499

IS - 2

ER -