Hypoxia-induced changes in insulin-like growth factors and their binding proteins in pregnant rats

Paivi J. Tapanainen, Peter Bang, Hermann L. Muller, Kristin Wilson, Ronald (Ron) Rosenfeld

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Pregnancy is associated with important changes in the insulin-like growth factor (IGF)-insulin-like growth factor binding protein (IGFBP) axis, but the importance of these growth factors for fetal growth is not well understood. We have recently established a maternal hypoxia model that results in significant intrauterine growth retardation in the fetus, and characterized the IGF-IGFBP axis in growth-retarded fetuses. To determine if maternal IGFs and their binding proteins are similarly regulated by hypoxia, we examined their expression in 6 hypoxic dams (13% oxygen, days 14-21 of gestation) and 6 control dams (21% oxygen). There was no significant difference in the food intake between the groups. The mean body weight of hypoxic dams, however, was 20% less than that of controls. Of all the organs, the lungs were most affected by hypoxia, weighing 17% more in the hypoxic dams than in the control dams; placental weight was reduced by 10% in the hypoxic dams. Liver and brain weights were not changed significantly by hypoxia. The mean concentration of immunoreactive IGF-I was 123 ± 11 ng/ml in the hypoxic dams and 130 ± 18 ng/ml in the control dams (nonsignificant). Similarly, there was no significant difference in hepatic IGF-I mRNA levels determined by solution hybridization nuclease-protection assay. An increase in IGFBP-1, IGFBP-2 and IGFBP-4 concentrations, however, could be observed by Western ligand blotting of the sera of hypoxic dams, compared to control dams. As assessed by Northern blot analysis, there was a 2.8-fold increase in IGFBP-1 mRNA expression in the livers of hypoxic dams compared to controls. Hepatic IGFBP-4 expression was also slightly increased (1.25-fold) in the hypoxic dams. No difference in hepatic IGFBP-2 or IGFBP-3 mRNA was found. Our results show parallel patterns in fetal and maternal IGF and IGFBP responses to hypoxia. This suggests that hypoxia may inhibit fetal growth by both directly affecting the fetus and via inhibition of placental growth.

Original languageEnglish (US)
Pages (from-to)227-234
Number of pages8
JournalHormone Research
Volume48
Issue number5
StatePublished - Nov 1997

Fingerprint

Insulin-Like Growth Factor Binding Proteins
Somatomedins
Insulin-Like Growth Factor Binding Protein 4
Liver
Insulin-Like Growth Factor Binding Protein 2
Insulin-Like Growth Factor Binding Protein 1
Fetus
Mothers
Fetal Development
Insulin-Like Growth Factor I
Messenger RNA
Nuclease Protection Assays
Oxygen
Weights and Measures
Pregnancy
Insulin-Like Growth Factor Binding Protein 3
Fetal Growth Retardation
Growth
Northern Blotting
Hypoxia

Keywords

  • Hypoxia
  • Insulin-like growth factor binding proteins
  • Insulin-like growth factors

ASJC Scopus subject areas

  • Endocrinology

Cite this

Tapanainen, P. J., Bang, P., Muller, H. L., Wilson, K., & Rosenfeld, R. R. (1997). Hypoxia-induced changes in insulin-like growth factors and their binding proteins in pregnant rats. Hormone Research, 48(5), 227-234.

Hypoxia-induced changes in insulin-like growth factors and their binding proteins in pregnant rats. / Tapanainen, Paivi J.; Bang, Peter; Muller, Hermann L.; Wilson, Kristin; Rosenfeld, Ronald (Ron).

In: Hormone Research, Vol. 48, No. 5, 11.1997, p. 227-234.

Research output: Contribution to journalArticle

Tapanainen, PJ, Bang, P, Muller, HL, Wilson, K & Rosenfeld, RR 1997, 'Hypoxia-induced changes in insulin-like growth factors and their binding proteins in pregnant rats', Hormone Research, vol. 48, no. 5, pp. 227-234.
Tapanainen PJ, Bang P, Muller HL, Wilson K, Rosenfeld RR. Hypoxia-induced changes in insulin-like growth factors and their binding proteins in pregnant rats. Hormone Research. 1997 Nov;48(5):227-234.
Tapanainen, Paivi J. ; Bang, Peter ; Muller, Hermann L. ; Wilson, Kristin ; Rosenfeld, Ronald (Ron). / Hypoxia-induced changes in insulin-like growth factors and their binding proteins in pregnant rats. In: Hormone Research. 1997 ; Vol. 48, No. 5. pp. 227-234.
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abstract = "Pregnancy is associated with important changes in the insulin-like growth factor (IGF)-insulin-like growth factor binding protein (IGFBP) axis, but the importance of these growth factors for fetal growth is not well understood. We have recently established a maternal hypoxia model that results in significant intrauterine growth retardation in the fetus, and characterized the IGF-IGFBP axis in growth-retarded fetuses. To determine if maternal IGFs and their binding proteins are similarly regulated by hypoxia, we examined their expression in 6 hypoxic dams (13{\%} oxygen, days 14-21 of gestation) and 6 control dams (21{\%} oxygen). There was no significant difference in the food intake between the groups. The mean body weight of hypoxic dams, however, was 20{\%} less than that of controls. Of all the organs, the lungs were most affected by hypoxia, weighing 17{\%} more in the hypoxic dams than in the control dams; placental weight was reduced by 10{\%} in the hypoxic dams. Liver and brain weights were not changed significantly by hypoxia. The mean concentration of immunoreactive IGF-I was 123 ± 11 ng/ml in the hypoxic dams and 130 ± 18 ng/ml in the control dams (nonsignificant). Similarly, there was no significant difference in hepatic IGF-I mRNA levels determined by solution hybridization nuclease-protection assay. An increase in IGFBP-1, IGFBP-2 and IGFBP-4 concentrations, however, could be observed by Western ligand blotting of the sera of hypoxic dams, compared to control dams. As assessed by Northern blot analysis, there was a 2.8-fold increase in IGFBP-1 mRNA expression in the livers of hypoxic dams compared to controls. Hepatic IGFBP-4 expression was also slightly increased (1.25-fold) in the hypoxic dams. No difference in hepatic IGFBP-2 or IGFBP-3 mRNA was found. Our results show parallel patterns in fetal and maternal IGF and IGFBP responses to hypoxia. This suggests that hypoxia may inhibit fetal growth by both directly affecting the fetus and via inhibition of placental growth.",
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