Hypotonic solution increases the slowly activating potassium current IsK expressed in Xenopus oocytes

Andreas E. Busch; Michael Varnum; John P. Adelman; R. Alan North

doi:10.1016/0006-291X(92)90661-4

Hypotonic solution increases the slowly activating potassium current I_sK expressed in Xenopus oocytes

Andreas E. Busch, Michael Varnum, John P. Adelman, R. Alan North

Vollum Institute

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

A slowly activating potassium current was expressed in Xenopus oocytes by injection of RNA transcribed from a rat kidney cDNA clone. Hypotonic solutions (160 mOsmol/l; control was 220 mOsmol/l) increased the current by increasing the rate of activation and by decreasing the depolarization needed to activate the current. This effect of hypotonicity was not observed in calcium-free solution, but was unaffected by staurosporine or the calmodulin antagonist W7. Cytochalasin D reduced the current and prevented the increase by hypotonic solution. The results suggest that the increase in this potassium current by hypotonic solution might result from calcium entry and changes in the actin network.

Original language	English (US)
Pages (from-to)	804-810
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	184
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(92)90661-4
State	Published - Apr 30 1992

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "A slowly activating potassium current was expressed in Xenopus oocytes by injection of RNA transcribed from a rat kidney cDNA clone. Hypotonic solutions (160 mOsmol/l; control was 220 mOsmol/l) increased the current by increasing the rate of activation and by decreasing the depolarization needed to activate the current. This effect of hypotonicity was not observed in calcium-free solution, but was unaffected by staurosporine or the calmodulin antagonist W7. Cytochalasin D reduced the current and prevented the increase by hypotonic solution. The results suggest that the increase in this potassium current by hypotonic solution might result from calcium entry and changes in the actin network.",

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AU - Busch, Andreas E.

AU - Varnum, Michael

AU - Adelman, John P.

AU - North, R. Alan

PY - 1992/4/30

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N2 - A slowly activating potassium current was expressed in Xenopus oocytes by injection of RNA transcribed from a rat kidney cDNA clone. Hypotonic solutions (160 mOsmol/l; control was 220 mOsmol/l) increased the current by increasing the rate of activation and by decreasing the depolarization needed to activate the current. This effect of hypotonicity was not observed in calcium-free solution, but was unaffected by staurosporine or the calmodulin antagonist W7. Cytochalasin D reduced the current and prevented the increase by hypotonic solution. The results suggest that the increase in this potassium current by hypotonic solution might result from calcium entry and changes in the actin network.

AB - A slowly activating potassium current was expressed in Xenopus oocytes by injection of RNA transcribed from a rat kidney cDNA clone. Hypotonic solutions (160 mOsmol/l; control was 220 mOsmol/l) increased the current by increasing the rate of activation and by decreasing the depolarization needed to activate the current. This effect of hypotonicity was not observed in calcium-free solution, but was unaffected by staurosporine or the calmodulin antagonist W7. Cytochalasin D reduced the current and prevented the increase by hypotonic solution. The results suggest that the increase in this potassium current by hypotonic solution might result from calcium entry and changes in the actin network.

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Hypotonic solution increases the slowly activating potassium current I_sK expressed in Xenopus oocytes

Abstract

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