Hypochlorite radioiodination of parathyroid peptides (hPTH1-34, [Tyr43]hPTH44-68)

Eric Orwoll, Ann Kopel, Zuzana Opsahl, Leslie Roberts, David Endres, Michael McClung

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Parathyroid hormone (PTH) radioimmunoassays have conventionally utilized [125I]bPTH1-84 as a radioligand, but more region-specific PTH assays are now possible with the use of recently available synthetic PTH peptides as standards and radioligands. A radioiodination procedure has been developed that utilizes hypochlorite as an oxidant and that is capable of producing PTH tracers of high specific activity (200 to 250 μCi/μg), prolonged stability, and excellent immunologic potency. Radioiodinated hPTH1-34 and [Tyr43]hPTH44-68 produced by hypochlorite iodination techniques can be used to develop sensitive and region-specific PTH assays of use in clinical and research situations.

Original languageEnglish (US)
Pages (from-to)239-246
Number of pages8
JournalThe Journal of Laboratory and Clinical Medicine
Volume105
Issue number2
StatePublished - Feb 1985

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Orwoll, E., Kopel, A., Opsahl, Z., Roberts, L., Endres, D., & McClung, M. (1985). Hypochlorite radioiodination of parathyroid peptides (hPTH1-34, [Tyr43]hPTH44-68). The Journal of Laboratory and Clinical Medicine, 105(2), 239-246.