Hypochlorite radioiodination of parathyroid peptides (hPTH1-34, [Tyr43]hPTH44-68)

Eric Orwoll; Ann Kopel; Zuzana Opsahl; Leslie Roberts; David Endres; Michael McClung

Hypochlorite radioiodination of parathyroid peptides (hPTH1-34, [Tyr43]hPTH44-68)

Eric Orwoll, Ann Kopel, Zuzana Opsahl, Leslie Roberts, David Endres, Michael McClung

Endocrinology

Research output: Contribution to journal › Article › peer-review

Abstract

Parathyroid hormone (PTH) radioimmunoassays have conventionally utilized [¹²⁵I]bPTH^1-84 as a radioligand, but more region-specific PTH assays are now possible with the use of recently available synthetic PTH peptides as standards and radioligands. A radioiodination procedure has been developed that utilizes hypochlorite as an oxidant and that is capable of producing PTH tracers of high specific activity (200 to 250 μCi/μg), prolonged stability, and excellent immunologic potency. Radioiodinated hPTH^1-34 and [Tyr⁴³]hPTH^44-68 produced by hypochlorite iodination techniques can be used to develop sensitive and region-specific PTH assays of use in clinical and research situations.

Original language	English (US)
Pages (from-to)	239-246
Number of pages	8
Journal	The Journal of Laboratory and Clinical Medicine
Volume	105
Issue number	2
State	Published - 1985

ASJC Scopus subject areas

Medicine(all)
Pathology and Forensic Medicine

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title = "Hypochlorite radioiodination of parathyroid peptides (hPTH1-34, [Tyr43]hPTH44-68)",

abstract = "Parathyroid hormone (PTH) radioimmunoassays have conventionally utilized [125I]bPTH1-84 as a radioligand, but more region-specific PTH assays are now possible with the use of recently available synthetic PTH peptides as standards and radioligands. A radioiodination procedure has been developed that utilizes hypochlorite as an oxidant and that is capable of producing PTH tracers of high specific activity (200 to 250 μCi/μg), prolonged stability, and excellent immunologic potency. Radioiodinated hPTH1-34 and [Tyr43]hPTH44-68 produced by hypochlorite iodination techniques can be used to develop sensitive and region-specific PTH assays of use in clinical and research situations.",

author = "Eric Orwoll and Ann Kopel and Zuzana Opsahl and Leslie Roberts and David Endres and Michael McClung",

year = "1985",

language = "English (US)",

volume = "105",

pages = "239--246",

journal = "Translational Research",

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T1 - Hypochlorite radioiodination of parathyroid peptides (hPTH1-34, [Tyr43]hPTH44-68)

AU - Orwoll, Eric

AU - Kopel, Ann

AU - Opsahl, Zuzana

AU - Roberts, Leslie

AU - Endres, David

AU - McClung, Michael

PY - 1985

Y1 - 1985

N2 - Parathyroid hormone (PTH) radioimmunoassays have conventionally utilized [125I]bPTH1-84 as a radioligand, but more region-specific PTH assays are now possible with the use of recently available synthetic PTH peptides as standards and radioligands. A radioiodination procedure has been developed that utilizes hypochlorite as an oxidant and that is capable of producing PTH tracers of high specific activity (200 to 250 μCi/μg), prolonged stability, and excellent immunologic potency. Radioiodinated hPTH1-34 and [Tyr43]hPTH44-68 produced by hypochlorite iodination techniques can be used to develop sensitive and region-specific PTH assays of use in clinical and research situations.

AB - Parathyroid hormone (PTH) radioimmunoassays have conventionally utilized [125I]bPTH1-84 as a radioligand, but more region-specific PTH assays are now possible with the use of recently available synthetic PTH peptides as standards and radioligands. A radioiodination procedure has been developed that utilizes hypochlorite as an oxidant and that is capable of producing PTH tracers of high specific activity (200 to 250 μCi/μg), prolonged stability, and excellent immunologic potency. Radioiodinated hPTH1-34 and [Tyr43]hPTH44-68 produced by hypochlorite iodination techniques can be used to develop sensitive and region-specific PTH assays of use in clinical and research situations.

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SN - 1931-5244

VL - 105

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JO - Translational Research

JF - Translational Research

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Hypochlorite radioiodination of parathyroid peptides (hPTH1-34, [Tyr43]hPTH44-68)

Abstract

ASJC Scopus subject areas

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