Hypochlorite radioiodination of parathyroid peptides (hPTH1-34, [Tyr43]hPTH44-68)

Eric Orwoll, Ann Kopel, Zuzana Opsahl, Leslie Roberts, David Endres, Michael McClung

Research output: Contribution to journalArticle

Abstract

Parathyroid hormone (PTH) radioimmunoassays have conventionally utilized [125I]bPTH1-84 as a radioligand, but more region-specific PTH assays are now possible with the use of recently available synthetic PTH peptides as standards and radioligands. A radioiodination procedure has been developed that utilizes hypochlorite as an oxidant and that is capable of producing PTH tracers of high specific activity (200 to 250 μCi/μg), prolonged stability, and excellent immunologic potency. Radioiodinated hPTH1-34 and [Tyr43]hPTH44-68 produced by hypochlorite iodination techniques can be used to develop sensitive and region-specific PTH assays of use in clinical and research situations.

Original languageEnglish (US)
Pages (from-to)239-246
Number of pages8
JournalThe Journal of Laboratory and Clinical Medicine
Volume105
Issue number2
StatePublished - 1985

Fingerprint

Hypochlorous Acid
Parathyroid Hormone
Peptides
Assays
Oxidants
Halogenation
Radioimmunoassay
Research

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Orwoll, E., Kopel, A., Opsahl, Z., Roberts, L., Endres, D., & McClung, M. (1985). Hypochlorite radioiodination of parathyroid peptides (hPTH1-34, [Tyr43]hPTH44-68). The Journal of Laboratory and Clinical Medicine, 105(2), 239-246.

Hypochlorite radioiodination of parathyroid peptides (hPTH1-34, [Tyr43]hPTH44-68). / Orwoll, Eric; Kopel, Ann; Opsahl, Zuzana; Roberts, Leslie; Endres, David; McClung, Michael.

In: The Journal of Laboratory and Clinical Medicine, Vol. 105, No. 2, 1985, p. 239-246.

Research output: Contribution to journalArticle

Orwoll, E, Kopel, A, Opsahl, Z, Roberts, L, Endres, D & McClung, M 1985, 'Hypochlorite radioiodination of parathyroid peptides (hPTH1-34, [Tyr43]hPTH44-68)', The Journal of Laboratory and Clinical Medicine, vol. 105, no. 2, pp. 239-246.
Orwoll, Eric ; Kopel, Ann ; Opsahl, Zuzana ; Roberts, Leslie ; Endres, David ; McClung, Michael. / Hypochlorite radioiodination of parathyroid peptides (hPTH1-34, [Tyr43]hPTH44-68). In: The Journal of Laboratory and Clinical Medicine. 1985 ; Vol. 105, No. 2. pp. 239-246.
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