Hypertonic saline injection model of experimental glaucoma in rats

John Morrison, Elaine Johnson, William O. Cepurna

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

A reliable method of creating chronic elevation of intraocular pressure (IOP) in rodents is an important tool in reproducing and studying the mechanisms of optic nerve injury that occur in glaucoma. In addition, such a model could provide a valuable method for testing potential neuroprotective treatments. This paper outlines the basic methods for producing obstruction of aqueous humor outflow and IOP elevation by injecting hypertonic saline (a sclerosant) into the aqueous outflow pathway. This is one of several rodent glaucoma models in use today. In this method, a plastic ring is placed around the equator of the eye to restrict injected saline to the limbus. By inserting a small glass microneedle in an aqueous outflow vein in the episclera and injecting hypertonic saline toward the limbus, the saline is forced into Schlemm’s canal and across the trabecular meshwork. The resultant inflammation and scarring of the anterior chamber angle occurs gradually, resulting in a rise in IOP after approximately 1 week. This article will describe the equipment necessary for producing this model and the steps of the technique itself.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages11-21
Number of pages11
Volume1695
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1695
ISSN (Print)1064-3745

Fingerprint

Glaucoma
Theoretical Models
Intraocular Pressure
Injections
Rodentia
Optic Nerve Injuries
Sclerosing Solutions
Trabecular Meshwork
Aqueous Humor
Anterior Chamber
Plastics
Cicatrix
Glass
Veins
Inflammation
Equipment and Supplies
Therapeutics

Keywords

  • Aqueous humor
  • Brown Norway rat
  • Chronic angle closure
  • Episcleral veins
  • Glaucoma
  • Hypertonic saline
  • Intraocular pressure
  • Optic nerve

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Morrison, J., Johnson, E., & Cepurna, W. O. (2018). Hypertonic saline injection model of experimental glaucoma in rats. In Methods in Molecular Biology (Vol. 1695, pp. 11-21). (Methods in Molecular Biology; Vol. 1695). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7407-8_2

Hypertonic saline injection model of experimental glaucoma in rats. / Morrison, John; Johnson, Elaine; Cepurna, William O.

Methods in Molecular Biology. Vol. 1695 Humana Press Inc., 2018. p. 11-21 (Methods in Molecular Biology; Vol. 1695).

Research output: Chapter in Book/Report/Conference proceedingChapter

Morrison, J, Johnson, E & Cepurna, WO 2018, Hypertonic saline injection model of experimental glaucoma in rats. in Methods in Molecular Biology. vol. 1695, Methods in Molecular Biology, vol. 1695, Humana Press Inc., pp. 11-21. https://doi.org/10.1007/978-1-4939-7407-8_2
Morrison J, Johnson E, Cepurna WO. Hypertonic saline injection model of experimental glaucoma in rats. In Methods in Molecular Biology. Vol. 1695. Humana Press Inc. 2018. p. 11-21. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7407-8_2
Morrison, John ; Johnson, Elaine ; Cepurna, William O. / Hypertonic saline injection model of experimental glaucoma in rats. Methods in Molecular Biology. Vol. 1695 Humana Press Inc., 2018. pp. 11-21 (Methods in Molecular Biology).
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