HyPer-3: A genetically encoded H2O2 probe with improved performance for ratiometric and fluorescence lifetime imaging

Dmitry S. Bilan; Luke Pase; Linda Joosen; Andrey Yu Gorokhovatsky; Yulia G. Ermakova; Theodorus W.J. Gadella; Clemens Grabher; Carsten Schultz; Sergey Lukyanov; Vsevolod V. Belousov

doi:10.1021/cb300625g

HyPer-3: A genetically encoded H₂O₂ probe with improved performance for ratiometric and fluorescence lifetime imaging

Dmitry S. Bilan, Luke Pase, Linda Joosen, Andrey Yu Gorokhovatsky, Yulia G. Ermakova, Theodorus W.J. Gadella, Clemens Grabher, Carsten Schultz, Sergey Lukyanov, Vsevolod V. Belousov

Research output: Contribution to journal › Article › peer-review

205 Scopus citations

Abstract

High-performance sensors for reactive oxygen species are instrumental to monitor dynamic events in cells and organisms. Here, we present HyPer-3, a genetically encoded fluorescent indicator for intracellular H₂O ₂ exhibiting improved performance with respect to response time and speed. HyPer-3 has an expanded dynamic range compared to HyPer and significantly faster oxidation/reduction dynamics compared to HyPer-2. We demonstrate this performance by in vivo imaging of tissue-scale H₂O₂ gradients in zebrafish larvae. Moreover, HyPer-3 was successfully employed for single-wavelength fluorescent lifetime imaging of H₂O₂ levels both in vitro and in vivo.

Original language	English (US)
Pages (from-to)	535-542
Number of pages	8
Journal	ACS chemical biology
Volume	8
Issue number	3
DOIs	https://doi.org/10.1021/cb300625g
State	Published - Mar 15 2013
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Medicine

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10.1021/cb300625g

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title = "HyPer-3: A genetically encoded H2O2 probe with improved performance for ratiometric and fluorescence lifetime imaging",

abstract = "High-performance sensors for reactive oxygen species are instrumental to monitor dynamic events in cells and organisms. Here, we present HyPer-3, a genetically encoded fluorescent indicator for intracellular H2O 2 exhibiting improved performance with respect to response time and speed. HyPer-3 has an expanded dynamic range compared to HyPer and significantly faster oxidation/reduction dynamics compared to HyPer-2. We demonstrate this performance by in vivo imaging of tissue-scale H2O2 gradients in zebrafish larvae. Moreover, HyPer-3 was successfully employed for single-wavelength fluorescent lifetime imaging of H2O2 levels both in vitro and in vivo.",

author = "Bilan, {Dmitry S.} and Luke Pase and Linda Joosen and Gorokhovatsky, {Andrey Yu} and Ermakova, {Yulia G.} and Gadella, {Theodorus W.J.} and Clemens Grabher and Carsten Schultz and Sergey Lukyanov and Belousov, {Vsevolod V.}",

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doi = "10.1021/cb300625g",

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AU - Bilan, Dmitry S.

AU - Pase, Luke

AU - Joosen, Linda

AU - Gorokhovatsky, Andrey Yu

AU - Ermakova, Yulia G.

AU - Gadella, Theodorus W.J.

AU - Grabher, Clemens

AU - Schultz, Carsten

AU - Lukyanov, Sergey

AU - Belousov, Vsevolod V.

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AB - High-performance sensors for reactive oxygen species are instrumental to monitor dynamic events in cells and organisms. Here, we present HyPer-3, a genetically encoded fluorescent indicator for intracellular H2O 2 exhibiting improved performance with respect to response time and speed. HyPer-3 has an expanded dynamic range compared to HyPer and significantly faster oxidation/reduction dynamics compared to HyPer-2. We demonstrate this performance by in vivo imaging of tissue-scale H2O2 gradients in zebrafish larvae. Moreover, HyPer-3 was successfully employed for single-wavelength fluorescent lifetime imaging of H2O2 levels both in vitro and in vivo.

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HyPer-3: A genetically encoded H₂O₂ probe with improved performance for ratiometric and fluorescence lifetime imaging

Abstract

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