HyPer-3: A genetically encoded H2O2 probe with improved performance for ratiometric and fluorescence lifetime imaging

Dmitry S. Bilan, Luke Pase, Linda Joosen, Andrey Yu Gorokhovatsky, Yulia G. Ermakova, Theodorus W.J. Gadella, Clemens Grabher, Carsten Schultz, Sergey Lukyanov, Vsevolod V. Belousov

Research output: Contribution to journalArticle

136 Scopus citations

Abstract

High-performance sensors for reactive oxygen species are instrumental to monitor dynamic events in cells and organisms. Here, we present HyPer-3, a genetically encoded fluorescent indicator for intracellular H2O 2 exhibiting improved performance with respect to response time and speed. HyPer-3 has an expanded dynamic range compared to HyPer and significantly faster oxidation/reduction dynamics compared to HyPer-2. We demonstrate this performance by in vivo imaging of tissue-scale H2O2 gradients in zebrafish larvae. Moreover, HyPer-3 was successfully employed for single-wavelength fluorescent lifetime imaging of H2O2 levels both in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)535-542
Number of pages8
JournalACS chemical biology
Volume8
Issue number3
DOIs
StatePublished - Mar 15 2013

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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    Bilan, D. S., Pase, L., Joosen, L., Gorokhovatsky, A. Y., Ermakova, Y. G., Gadella, T. W. J., Grabher, C., Schultz, C., Lukyanov, S., & Belousov, V. V. (2013). HyPer-3: A genetically encoded H2O2 probe with improved performance for ratiometric and fluorescence lifetime imaging. ACS chemical biology, 8(3), 535-542. https://doi.org/10.1021/cb300625g