Hydration with saline decreases toxicity of mice injected with calcitriol in preclinical studies

Amir A. Azari, Mozhgan R. Kanavi, Soesiawati R. Darjatmoko, Vivian Lee, Kyung Mann Kim, Heather D. Potter, Daniel M. Albert

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The effectiveness of saline injection in reducing the toxicity profile of calcitriol when coadministered in mice was evaluated. Mortality was used as an end point to study the toxic effects of calcitriol; the relative risk of mortality in mice injected with saline was evaluated from our previously published animal experiments. We discovered that coadministration with 0.25 mL normal saline solution injected intraperitoneally is associated with a lower mortality rate than calcitriol given alone. The estimated relative risk of mortality was 0.0789 (95% confidence interval, 0.0051-1.22; z = 1.82; P = 0.070) when saline is administered with calcitriol compared to calcitriol alone. There was a reduction in serum calcium levels in mice that received saline (11.4 ± 0.15 mg/dL) compared to mice that did not receive saline (12.42 ± 1.61 mg/dL). Hydration with saline seems to reduce mortality and toxicity in mice receiving calcitriol. Given the decrease in mortality rates, intraperitoneal injections of saline should be considered in studies involving mice receiving injections of calcitriol.

Original languageEnglish (US)
Pages (from-to)241-244
Number of pages4
JournalJournal of Environmental Pathology, Toxicology and Oncology
Volume32
Issue number3
DOIs
StatePublished - Nov 12 2013

Keywords

  • Calcitriol
  • Saline hydration
  • Toxicity

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Health, Toxicology and Mutagenesis

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