Hydration with saline decreases toxicity of mice injected with calcitriol in preclinical studies

Amir A. Azari, Mozhgan R. Kanavi, Soesiawati R. Darjatmoko, Vivian Lee, Kyung Mann Kim, Heather D. Potter, Daniel Albert

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The effectiveness of saline injection in reducing the toxicity profile of calcitriol when coadministered in mice was evaluated. Mortality was used as an end point to study the toxic effects of calcitriol; the relative risk of mortality in mice injected with saline was evaluated from our previously published animal experiments. We discovered that coadministration with 0.25 mL normal saline solution injected intraperitoneally is associated with a lower mortality rate than calcitriol given alone. The estimated relative risk of mortality was 0.0789 (95% confidence interval, 0.0051-1.22; z = 1.82; P = 0.070) when saline is administered with calcitriol compared to calcitriol alone. There was a reduction in serum calcium levels in mice that received saline (11.4 ± 0.15 mg/dL) compared to mice that did not receive saline (12.42 ± 1.61 mg/dL). Hydration with saline seems to reduce mortality and toxicity in mice receiving calcitriol. Given the decrease in mortality rates, intraperitoneal injections of saline should be considered in studies involving mice receiving injections of calcitriol.

Original languageEnglish (US)
Pages (from-to)241-244
Number of pages4
JournalJournal of Environmental Pathology, Toxicology and Oncology
Volume32
Issue number3
DOIs
StatePublished - Nov 12 2013
Externally publishedYes

Fingerprint

Calcitriol
Hydration
Toxicity
Mortality
Injections
Poisons
Intraperitoneal Injections
Sodium Chloride
Animals
Confidence Intervals
Calcium
Serum

Keywords

  • Calcitriol
  • Saline hydration
  • Toxicity

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Hydration with saline decreases toxicity of mice injected with calcitriol in preclinical studies. / Azari, Amir A.; Kanavi, Mozhgan R.; Darjatmoko, Soesiawati R.; Lee, Vivian; Kim, Kyung Mann; Potter, Heather D.; Albert, Daniel.

In: Journal of Environmental Pathology, Toxicology and Oncology, Vol. 32, No. 3, 12.11.2013, p. 241-244.

Research output: Contribution to journalArticle

Azari, Amir A. ; Kanavi, Mozhgan R. ; Darjatmoko, Soesiawati R. ; Lee, Vivian ; Kim, Kyung Mann ; Potter, Heather D. ; Albert, Daniel. / Hydration with saline decreases toxicity of mice injected with calcitriol in preclinical studies. In: Journal of Environmental Pathology, Toxicology and Oncology. 2013 ; Vol. 32, No. 3. pp. 241-244.
@article{30a1ebd4accd439f8234e7197462a1c2,
title = "Hydration with saline decreases toxicity of mice injected with calcitriol in preclinical studies",
abstract = "The effectiveness of saline injection in reducing the toxicity profile of calcitriol when coadministered in mice was evaluated. Mortality was used as an end point to study the toxic effects of calcitriol; the relative risk of mortality in mice injected with saline was evaluated from our previously published animal experiments. We discovered that coadministration with 0.25 mL normal saline solution injected intraperitoneally is associated with a lower mortality rate than calcitriol given alone. The estimated relative risk of mortality was 0.0789 (95{\%} confidence interval, 0.0051-1.22; z = 1.82; P = 0.070) when saline is administered with calcitriol compared to calcitriol alone. There was a reduction in serum calcium levels in mice that received saline (11.4 ± 0.15 mg/dL) compared to mice that did not receive saline (12.42 ± 1.61 mg/dL). Hydration with saline seems to reduce mortality and toxicity in mice receiving calcitriol. Given the decrease in mortality rates, intraperitoneal injections of saline should be considered in studies involving mice receiving injections of calcitriol.",
keywords = "Calcitriol, Saline hydration, Toxicity",
author = "Azari, {Amir A.} and Kanavi, {Mozhgan R.} and Darjatmoko, {Soesiawati R.} and Vivian Lee and Kim, {Kyung Mann} and Potter, {Heather D.} and Daniel Albert",
year = "2013",
month = "11",
day = "12",
doi = "10.1615/JEnvironPatholToxicolOncol.2013007532",
language = "English (US)",
volume = "32",
pages = "241--244",
journal = "Journal of Environmental Pathology, Toxicology and Oncology",
issn = "0731-8898",
publisher = "Begell House Inc.",
number = "3",

}

TY - JOUR

T1 - Hydration with saline decreases toxicity of mice injected with calcitriol in preclinical studies

AU - Azari, Amir A.

AU - Kanavi, Mozhgan R.

AU - Darjatmoko, Soesiawati R.

AU - Lee, Vivian

AU - Kim, Kyung Mann

AU - Potter, Heather D.

AU - Albert, Daniel

PY - 2013/11/12

Y1 - 2013/11/12

N2 - The effectiveness of saline injection in reducing the toxicity profile of calcitriol when coadministered in mice was evaluated. Mortality was used as an end point to study the toxic effects of calcitriol; the relative risk of mortality in mice injected with saline was evaluated from our previously published animal experiments. We discovered that coadministration with 0.25 mL normal saline solution injected intraperitoneally is associated with a lower mortality rate than calcitriol given alone. The estimated relative risk of mortality was 0.0789 (95% confidence interval, 0.0051-1.22; z = 1.82; P = 0.070) when saline is administered with calcitriol compared to calcitriol alone. There was a reduction in serum calcium levels in mice that received saline (11.4 ± 0.15 mg/dL) compared to mice that did not receive saline (12.42 ± 1.61 mg/dL). Hydration with saline seems to reduce mortality and toxicity in mice receiving calcitriol. Given the decrease in mortality rates, intraperitoneal injections of saline should be considered in studies involving mice receiving injections of calcitriol.

AB - The effectiveness of saline injection in reducing the toxicity profile of calcitriol when coadministered in mice was evaluated. Mortality was used as an end point to study the toxic effects of calcitriol; the relative risk of mortality in mice injected with saline was evaluated from our previously published animal experiments. We discovered that coadministration with 0.25 mL normal saline solution injected intraperitoneally is associated with a lower mortality rate than calcitriol given alone. The estimated relative risk of mortality was 0.0789 (95% confidence interval, 0.0051-1.22; z = 1.82; P = 0.070) when saline is administered with calcitriol compared to calcitriol alone. There was a reduction in serum calcium levels in mice that received saline (11.4 ± 0.15 mg/dL) compared to mice that did not receive saline (12.42 ± 1.61 mg/dL). Hydration with saline seems to reduce mortality and toxicity in mice receiving calcitriol. Given the decrease in mortality rates, intraperitoneal injections of saline should be considered in studies involving mice receiving injections of calcitriol.

KW - Calcitriol

KW - Saline hydration

KW - Toxicity

UR - http://www.scopus.com/inward/record.url?scp=84887178742&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887178742&partnerID=8YFLogxK

U2 - 10.1615/JEnvironPatholToxicolOncol.2013007532

DO - 10.1615/JEnvironPatholToxicolOncol.2013007532

M3 - Article

C2 - 24266410

AN - SCOPUS:84887178742

VL - 32

SP - 241

EP - 244

JO - Journal of Environmental Pathology, Toxicology and Oncology

JF - Journal of Environmental Pathology, Toxicology and Oncology

SN - 0731-8898

IS - 3

ER -