Hydration and elevated calcium alone do not produce xylose nuclear cataract: Role of proteolysis by calpain

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Abstract

The purpose of this experiment was to determine the contribution of calpain proteolytic enzyme (EC 3.4.22.17) in the formation of nuclear cataract during lens culture in xylose. Increased lens calcium was found to be required for formation of xylose nuclear cataract in our culture system. Inhibition of calpain by the cysteine protease inhibitor E64 was effective in slowing the formation of nuclear cataract, even though lens calcium and hydration were markedly elevated. These results showed that hydration and elevated calcium alone do not produce xylose nuclear cataract, and they indicated that calpain proteolysis may be necessary for xylose nuclear cataract in the rat lens.

Original languageEnglish (US)
Pages (from-to)8-14
Number of pages7
JournalOphthalmic Research
Volume24
Issue number1
DOIs
StatePublished - Jan 1 1992

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Keywords

  • Calcium
  • Calpain
  • Cataract
  • Culture
  • E64
  • Xylose

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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